Autonomic brainstem nuclei are linked to the hippocampus
Autonomic brainstem nuclei are linked to the hippocampus
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DOI：
10.1016/j.neuroscience.2005.04.031
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Loewy, AD
中科院分区：
文献类型：
--
作者：
Castle, M;Comoli, E;Loewy, AD
据报道，迷走神经刺激可增强大鼠和人类的记忆力，并可有效治疗某些患者的癫痫，但导致这些效应的潜在神经解剖学基质仍不清楚。由于从孤束核（大脑的主要迷走神经中继部位）到海马体没有直接的解剖学投射，我们测试了是否存在多突触通路。伪狂犬病病毒，猪疱疹病毒，可作为一种逆行transneuronal示踪剂，被注射到腹侧CA 1海马的大鼠，4天后，伪狂犬病病毒感染的神经元被确定在一般内脏部分的孤束核，与大多数被定位在A2去甲肾上腺素能细胞群。其他自主脑干核团，包括臂旁核，蓝斑，A1和A5去甲肾上腺素能细胞群，和C1肾上腺素能细胞群，被标记。为了确定孤束核-海马通路的一些潜在中继位点，腹侧CA 1区的免疫毒素损伤选择性地破坏去甲肾上腺素能或胆碱能纤维。恢复2周后，在同一CA 1区注射伪狂犬病病毒，4天后，孤束核中的跨神经元标记减少约65%。这些结果表明，蓝斑的去甲肾上腺素能神经元和内侧隔/斜角带的胆碱能神经元可能是该通路的中继站点。还讨论了其他可能的联系。总之，这是第一个解剖报告，表明一般内脏区的孤束核是通过多突触中继海马。(c)2005年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Vagal nerve stimulation has been reported to enhance memory in both rats and humans, and to be an effective treatment for epilepsy in some patients, but the underlying neuroanatomical substrate(s) responsible for these effects remains unknown. Since there is no direct anatomical projection from the nucleus tractus solitarius, the main vagal relay site of the brain, to the hippocampus, we tested whether a multisynaptic pathway exists. Pseudorabies virus, a pig herpesvirus that can be used as a retrograde transneuronal tracer, was injected into the ventral CA1 hippocampus of rats, and after 4 days, pseudorabies virus infected neurons were identified in the general visceral portion of the nucleus tractus solitarius, with the majority being localized in the A2 noradrenergic cell group. Other autonomic brainstem nuclei, including the parabrachial nucleus, locus coeruleus, A1 and A5 noradrenergic cell groups, and C1 adrenergic cell group, were labeled. In order to identify some of the potential relay sites of the nucleus tractus solitarius-hippocampal pathway, immunotoxin lesions of the ventral CA1 region were made that selectively destroyed either the noradrenergic or cholinergic fibers. After 2 weeks' recovery, pseudorabies virus was injected in this same CA1 area, and 4 days later, the transneuronal labeling in the nucleus tractus solitarius was reduced by approximately 65%. These findings suggest that the noradrenergic neurons of the locus coeruleus and cholinergic neurons of the medial septum/diagonal band are likely to be relay sites for this pathway. Other potential linkages are discussed. In summary, this is the first anatomical report to show that the general visceral region of nucleus tractus solitarius is linked via multisynaptic relays to the hippocampus. (c) 2005 IBRO. Published by Elsevier Ltd. All rights reserved.