Ion fluxes across the pitcher walls of three Bornean Nepenthes pitcher plant species: flux rates and gland distribution patterns reflect nitrogen sequestration strategies.
Ion fluxes across the pitcher walls of three Bornean Nepenthes pitcher plant species: flux rates and gland distribution patterns reflect nitrogen sequestration strategies.
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DOI：
10.1093/jxb/erq004
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发表时间：
2010-03
影响因子：
6.9
通讯作者：
Robbins SL
中科院分区：
文献类型：
--
作者：
Moran JA;Hawkins BJ;Gowen BE;Robbins SL
猪笼草的捕虫笼植物种类在猎物捕获策略、猎物捕获率和捕虫笼寿命方面有所不同。在本研究中，研究了养分封存策略的种间差异是否反映在猪笼草本身的生理和微观结构中。使用非侵入性技术（MIFE）测量了壶腹猪笼草、二齿猪笼草 Hook.f. 和莱佛士猪笼草的捕虫笼中的离子通量。扫描电子显微镜也用于表征腺体和其他结构在内笼壁上的分布。结果表明，养分封存策略确实反映在猪笼的生理学和微观结构中。产生长寿命捕虫笼且猎物捕获率低的物种（壶腹猪笼草、二齿猪笼草）的 NH4+ 吸收率低于莱佛士猪笼草（捕虫笼寿命短、捕获率高）。至关重要的是，依赖于水生共生生物（壶腹猪笼草、二齿猪笼草）的物种主动操纵 H+ 通量，以维持比“典型”物种更少的酸性捕虫笼液；此外，这些物种缺乏“典型”食虫同类所特有的月骨细胞和表皮蜡质。一个意想不到的发现是，莱佛士猪笼草的蜡覆盖的非腺体区域发生了离子通量。在这些区域中主动运输水性离子的唯一候选者似乎是位于月骨细胞下方的表皮细胞，因为这些是唯一没有明显涂覆表皮蜡的部位。
Nepenthes pitcher plant species differ in their prey capture strategies, prey capture rates, and pitcher longevity. In this study, it is investigated whether or not interspecific differences in nutrient sequestration strategy are reflected in the physiology and microstructure of the pitchers themselves. Using a non-invasive technique (MIFE), ion fluxes in pitchers of Nepenthes ampullaria Jack, Nepenthes bicalcarata Hook.f., and Nepenthes rafflesiana Jack were measured. Scanning electron microscopy was also used to characterize the distribution of glandular and other structures on the inner pitcher walls. The results demonstrate that nutrient sequestration strategy is indeed mirrored in pitcher physiology and microstructure. Species producing long-lived pitchers with low prey capture rates (N. ampullaria, N. bicalcarata) showed lower rates of NH4+ uptake than N. rafflesiana, a species producing short-lived pitchers with high capture rates. Crucially, species dependent upon aquatic commensals (N. ampullaria, N. bicalcarata) actively manipulated H+ fluxes to maintain less acid pitcher fluid than found in ‘typical’ species; in addition, these species lacked the lunate cells and epicuticular waxes characteristic of ‘typical’ insectivorous congeners. An unexpected finding was that ion fluxes occurred in the wax-covered, non-glandular zones in N. rafflesiana. The only candidates for active transport of aqueous ions in these zones appear to be the epidermal cells lying beneath the lunate cells, as these are the only sites not visibly coated with epicuticular waxes.
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影响因子：
2.2
作者：
CLARKE, CM;KITCHING, RL
通讯作者：
KITCHING, RL
影响因子：
9.4
作者：
Gaume, L;Gorb, S;Rowe, N
通讯作者：
Rowe, N
影响因子：
2.9
作者：
Di Giusto, Bruno;Grosbois, Vladimir;Gaume, Laurence
通讯作者：
Gaume, Laurence
影响因子：
4.3
作者：
An, CI;Takekawa, S;Kobayashi, A
通讯作者：
Kobayashi, A
影响因子：
1.6
作者：
DRING J
通讯作者：
DRING J