Production of gynogenetic progeny from allotetraploid hybrids red crucian carp x common carp
Production of gynogenetic progeny from allotetraploid hybrids red crucian carp x common carp
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红鲫×鲤异源四倍体杂交后代的产生
DOI：
10.1016/j.aquaculture.2003.10.001
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发表时间：
2004-06-01
期刊：
影响因子：
4.5
通讯作者：
Liu, Y
中科院分区：
文献类型：
--
作者：
Liu, SJ;Sun, YD;Liu, Y
经紫外线照射的精子激活后，异源四倍体杂交种(红鲤雌性×鲤鱼雄性)的二倍体卵子发育成正常的活体雌性激素。这些冷休克激活的二倍体卵子未能诱导出预期的四倍体。所有这些雌核发育后代，无论有没有冷休克，都是雌性二倍体，有100条染色体，表明四倍体雌性的XXXX基因型。雌核发育雌体在2+龄成熟，与二倍体雌体相比，成熟延迟。30%的雌核发育良好，卵巢正常，卵子成熟。这些雌核发育基因在形态上与F-2杂交种没有区别。与二倍体F-2杂交种一样，这些二倍体雌核发育成二倍体卵子，当这些卵子与红鲤杂交时，这些卵子发育成具有150条染色体的三倍体。在不进行染色体倍增处理的情况下，由F-1雌雄配子产生的二倍体卵子经紫外线照射的精子激活后发育成F-2雌核配子。这些二倍体卵子的产生可能是减数分裂前染色体内复制的结果。在水产养殖中，这些二倍体卵子仍然是产生四倍体和三倍体以及建立二倍体雌核发育杂交系的重要配子来源。(C)2003爱思唯尔B.V.保留所有权利。
Following activation by UV-irradiated sperm, diploid eggs of the allotetraploid hybrids (red crucian carp female x common carp male) developed into normal live gynogens. Cold-shocking these activated diploid eggs failed to induce the expected tetraploidy in them. All these gynogenetic progenies, generated with or without the cold shock, were female diploids with 100 chromosomes, indicating the XXXX genotype of the tetraploid females. The gynogenetic females matured at the age of 2+ suggesting delayed maturity, in comparison to the diploid females of the red crucian carp. Thirty percent of these gynogens were fertile with normal ovary and mature eggs. These gynogens were not morphologically distinguishable from the F-2 hybrids. Like the diploid F-2 hybrids, these diploid gynogens produced diploid eggs, for these eggs developed into triploids with 150 chromosomes, when crossed with red crucian carp. Without the treatment for doubling the chromosome number, the diploid eggs, produced by the F-1 gynogens, developed into the F-2 gynogens following activation by UV-irradiated sperm. Production of these diploid eggs from the diploid gynogens may have resulted from the pre-meiotic endoreduplication of chromosomes. For aquaculture, these diploid eggs remain an important gamete source for the production of tetraploids and triploids, and establishment of diploid gynogenetic hybrid line. (C) 2003 Elsevier B.V. All rights reserved.