BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION .2. KINETICS OF NUCLEIC ACID AND PROTEIN SYNTHESIS IN SUSPENSION CULTURES
BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION .2. KINETICS OF NUCLEIC ACID AND PROTEIN SYNTHESIS IN SUSPENSION CULTURES
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DOI：
10.1016/0042-6822(61)90051-4
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发表时间：
1961-01-01
期刊：
影响因子：
3.7
通讯作者：
DAESCH, GE
中科院分区：
文献类型：
--
作者：
GREEN, M;DAESCH, GE
用2型腺病毒在高输入倍数下感染悬浮中呈指数增长的KB细胞。在感染后14小时首次检测到细胞内病毒增加，在感染后36-48小时达到每个细胞4000-10,000 PFU的水平。在48小时的实验期间，未感染的细胞增殖3至4倍，相比之下，在感染的培养物中，细胞的增殖仅限于前12小时，从未超过1.2至1.3倍。病毒吸附、感染中心形成和存活细胞的测量表明，基本上所有细胞在加入病毒后1小时内都被感染。被感染的细胞没有溶解，但看起来体积增大了。干重分析显示，感染后36小时，每个感染细胞的质量持续增加，是对照细胞的两倍。蛋白质、DNA和RNA在感染后12小时内开始在感染细胞中持续积累。在感染后36小时，每个细胞中这些聚合物的含量达到未感染细胞的两倍。讨论了增加聚合物合成对病毒产生的意义。
KB cells growing exponen-tially in suspension were infected with type 2 adenovirus at high input multiplicities. An increase in intracellular virus was first detected at 14 hours after infection and reached levels of 4000-10,000 PFU per cell at 36-48 hours after infection. Uninfected cells multiplied 3- to 4-fold during 48-hour experimental periods, In contrast, multiplication of cells in infected cultures was limited to the first 12 hours and was never more than 1.2- to 1.3-fold. Measurements of virus adsorption, infective center formation, and surviving cells indicated that essentially all cells were infected within 1 hour after addition of virus. Infected cells did not lyse but appeared to increase in size. Dry weight analysis revealed a continuous increase in mass per infected cell to double that of control cells at 36 hours after infection. Protein, DNA, and RNA accumulated continuously in infected cells starting within 12 hours after infection. At 36 hours after infection the content per cell of these polymers reached double that of uninfected cells. The significance of increased polymer synthesis in relation to virus production is discussed.