The effects of the plasminogen pathway on scar tissue formation.
The effects of the plasminogen pathway on scar tissue formation.
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纤溶酶原途径对疤痕组织形成的影响。
DOI：
10.1097/00005537-200401000-00007
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Nguyen,Hoang-LanT
中科院分区：
文献类型：
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作者：
Samara,GhassanJ;Schaffner,AdamD;Eisenstat,Jonathan;Nguyen,Hoang-LanT
目的/假设作者试图确定纤溶酶原途径在伤口愈合中的作用。他们假设纤维蛋白降解减少可能导致胶原沉积增加。假设组织病理学异常的程度与疤痕的美学外观相关，我们进行了一项研究，试图确定有和没有纤溶酶原途径功能受损的小鼠中疤痕组织的组织病理学外观。纤溶酶原途径在伤口愈合中的作用进行了研究，通过分析瘢痕组织的形成，使用所述的methods.MethodsA 2厘米的切口上的背部的小鼠有和没有特定的遗传缺陷的纤溶酶原途径。动物处死后，收获组织，固定，并使用苏木精和伊红以及三色染色制备。由两名独立的研究者以盲法进行组织学分析和评分。Student'sttest用于确定组间的统计学显著性。ResultsA统计学显著性差异胶原蛋白取向与纤溶酶原通路功能受损的小鼠和野生型（对照）组（P= 0.0163）。纤溶酶原缺陷小鼠的总体组织形态学评分（P= 0.0706）显示出改善伤口愈合的统计学趋势（P = 0.0706）。因此，应进一步研究其他溶栓和抗溶栓药物在伤口愈合中的作用，以准确识别在瘢痕组织形成中发挥最重要作用的药物。
Objectives/HypothesisThe authors sought to determine the role of the plasminogen pathway in wound healing. They hypothesized that decreased fibrin degradation may lead to increased collagen deposition. Presuming that the degree of histopathological abnormality correlates with the aesthetic appearance of the scar, we conducted a study that attempted to determine the histopathological appearance of scar tissue in mice with and without impaired function of the plasminogen pathway.Study DesignMice with and without deficiencies in the plasminogen pathway underwent surgery. The role of the plasminogen pathway in wound healing was studied by analysis of scar tissue formation using the methods described.MethodsA 2‐cm incision was made on the dorsum of mice with and without specified genetic deficiencies in the plasminogen pathway. After the animals were killed, the tissue was harvested, fixed, and prepared using hematoxylin and eosin as well as trichrome stains. Histopathological analysis and scoring were performed by two separate investigators in a blinded manner. Student'sttest was used to determine statistical significance between groups.ResultsA statistically significant difference in collagen orientation was noted between mice with impaired plasminogen pathway function and the wild‐type (control) group (P= .0163). A statistical trend toward improved wound healing for plasminogen‐deficient mice was found for overall histomorphological score (P= .0706).ConclusionThe role of the plasminogen pathway in wound healing is one that should be noted and may lead to the development of new therapies that reduce scar tissue formation. Hence, the role of other thrombolytic and anti‐thrombolytic agents in wound healing should be further investigated to precisely identify agents that play the most significant role in scar tissue formation.