Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.
Small interfering RNA inhibits SARS-CoV nucleocapsid gene expression in cultured cells and mouse muscles.
复制标题
小型干扰RNA抑制培养细胞和小鼠肌肉中的SARS-COV核素基因表达。
DOI：
10.1016/j.febslet.2005.02.080
复制
发表时间：
2005-04-25
期刊：
影响因子：
3.5
通讯作者：
Qi ZT
中科院分区：
文献类型：
--
作者：
Zhao P;Qin ZL;Ke JS;Lu Y;Liu M;Pan W;Zhao LJ;Cao J;Qi ZT
SARS-CoV是一种新发现的冠状病毒，可引起严重急性呼吸综合征（SARS）。目前，还没有有效的方法可用于预防和治疗SARS-CoV感染。在本研究中，小干扰RNA（siRNA）对SARS冠状病毒核衣壳（N）蛋白表达的影响在培养细胞和小鼠肌肉中进行了检测。制备了4个由小鼠U6启动子驱动的针对SARS冠状病毒N基因的siRNA表达盒，观察其对N与增强型绿色荧光蛋白（EGFP）融合蛋白表达的抑制作用。候选siRNA被证明以序列特异性方式主动下调N和EGFP表达。构建了该siRNA的表达载体，并证实其在培养细胞和成年小鼠肌肉中均有效降低N和EGFP表达。我们的研究结果表明，siRNA为预防和治疗人类SARS-CoV感染提供了基础。
SARS‐CoV is a newly identified coronavirus that causes severe acute respiratory syndrome (SARS). Currently, there is no effective method available for prophylaxis and treatment of SARS‐CoV infections. In the present study, the influence of small interfering RNA (siRNA) on SARS‐CoV nucleocapsid (N) protein expression was detected in cultured cells and mouse muscles. Four siRNA expression cassettes driven by mouse U6 promoter targeting SARS‐CoV N gene were prepared, and their inhibitory effects on expression of N and enhanced green fluorescence protein (EGFP) fusion protein were observed. A candidate siRNA was proved to down‐regulate N and EGFP expression actively in a sequence‐specific manner. The expression vector of this siRNA was constructed and confirmed to reduce N and EGFP expression efficiently in both cultured cells and adult mouse muscles. Our findings suggest that the siRNA should provide the basis for prophylaxis and therapy of SARS‐CoV infection in human.
登录
查看更多内容
影响因子：
64.8
作者：
Soutschek, J;Akinc, A;Vornlocher, HP
通讯作者：
Vornlocher, HP
影响因子：
46.9
作者：
Kennerdell, JR;Carthew, RW
通讯作者：
Carthew, RW
影响因子：
4.5
作者：
Castanotto, D;Li, HT;Rossi, JJ
通讯作者：
Rossi, JJ
影响因子：
11.8
作者：
Vabret, A;Mourez, T;Freymuth, F
通讯作者：
Freymuth, F
影响因子：
3.5
作者：
Gou, DM;Jin, NL;Liu, L
通讯作者：
Liu, L