RAPID PROCEDURE FOR ISOLATION OF PLASMID DNA AND APPLICATION TO EPIDEMIOLOGICAL ANALYSIS
RAPID PROCEDURE FOR ISOLATION OF PLASMID DNA AND APPLICATION TO EPIDEMIOLOGICAL ANALYSIS
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DOI：
10.1128/jcm.20.4.608-613.1984
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发表时间：
1984-01-01
影响因子：
9.4
通讯作者：
NAGANO, Y
中科院分区：
文献类型：
--
作者：
TAKAHASHI, S;NAGANO, Y
建立了一种快速、简便的质粒分离方法，用于质粒介导的耐药性流行病学分析。通过这种方法，可获得分子量在2.0和122 × 104之间的质粒DNA。106可以检测。可以分析各种细菌，例如肠杆菌科、铜绿假单胞菌、流感嗜血杆菌和金黄色葡萄球菌的菌株。获得的质粒DNA无需进一步纯化即可直接用于限制性内切酶分析。这种方法可以同时分析几种培养物。
A rapid and simple plasmid isolation procedure was developed for the epidemiological analysis of plasmid-mediated antimicrobial resistance. By this method, plasmid DNA ranging in MW between 2.0 and 122 .times. 106 could be detected. Various bacteria, such as strains of the family Enterobacteriaceae, Pseudomonas aeruginosa, Haemophilus influenzae and Staphylococcus aureus, could be analyzed. The plasmid DNA obtained could be directly used for restriction endonuclease analysis without further purification. This method made it possible to analyze several cultures at the same time.