Quantitative ToF-SIMS Studies of Protein Drug Release from Biodegradable Polymer Drug Delivery Membranes.
Quantitative ToF-SIMS Studies of Protein Drug Release from Biodegradable Polymer Drug Delivery Membranes.
复制标题
可生物降解聚合物药物递送膜中蛋白质药物释放的定量 ToF-SIMS 研究。
DOI：
10.1016/j.apsusc.2008.05.082
复制
发表时间：
2008
影响因子：
6.7
通讯作者：
Gardella,JosephA
中科院分区：
文献类型：
--
作者：
Burns,SarahA;Gardella,JosephA
生物可降解聚合物在开发控制蛋白质药物递送的策略中是令人感兴趣的。本研究中使用的蛋白质是角质细胞生长因子（KGF），其是参与上皮再形成过程的蛋白质。在配制过程中和聚合物降解过程中，使用离子表面活性剂Aerosol-OT（AOT）将蛋白质包封在水性环境中，使蛋白质稳定在可生物降解的聚合物基质中。蛋白质从这些材料表面的释放动力学需要精确的时间，这是该药物递送系统功效的关键因素。飞行时间二次离子质谱法（ToF-SIMS）用于相同的能力，以确定表面活性剂和聚合物的分子离子峰，并使用它来确定表面浓度。在聚合物基质中，分别在m/z 467和421处以正模式和负模式观察到表面活性剂分子离子峰。这些峰被确定为[AOT+Na+]和[AOT−Na+]。这些方法用于从聚合物基质中鉴别表面活性剂和蛋白质，并用于测量表面积聚速率。第二步是将该积累速率与在生物可降解膜降解期间蛋白质释放到水溶液中的速率进行比较。将该速率与来自荧光光谱测量的速率进行比较，所述荧光光谱测量使用来自释放到水溶液中的蛋白质的自发荧光[C.M. Mahoney，J. Yu，A. Fahey，J. A. J. Gardella，聚合物与基于蛋白质的药物共混物的西姆斯深度剖析，Appl. Surf. Sci. 252（2006），6609-6614.]。
Biodegradable polymers are of interest in developing strategies to control protein drug delivery. The protein that was used in this study is Keratinocyte Growth Factor (KGF) which is a protein involved in the re-epithelialization process. The protein is stabilized in the biodegradable polymer matrix during formulation and over the course of polymer degradation with the use of an ionic surfactant Aerosol-OT (AOT) which will encapsulate the protein in an aqueous environment. The release kinetics of the protein from the surface of these materials requires precise timing which is a crucial factor in the efficacy of this drug delivery system. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) was used in the same capacity to identify the molecular ion peak of the surfactant and polymer and use this to determine surface concentration. In the polymer matrix, the surfactant molecular ion peak was observed in the positive and negative mode at m/z 467 and 421, respectively. These peaks were determined to be [AOT+Na+] and [AOT−Na+]. These methods are used to identify the surfactant and protein from the polymer matrix and are used to measure the rate of surface accumulation. The second step was to compare this accumulation rate with the release rate of the protein into an aqueous solution during the degradation of the biodegradable film. This rate is compared to that from fluorescence spectroscopy measurements using the protein autofluorescence from that released into aqueous solution [C.M. Mahoney, J. Yu, A. Fahey, J.A.J. Gardella, SIMS depth profiling of polymer blends with protein based drugs, Appl. Surf. Sci. 252 (2006), 6609–6614.].