Brain-derived neurotrophic factor-, neurotrophin-3-, and tyrosine kinase receptor-like immunoreactivity in lingual taste bud fields of mature hamster after sensory denervation
Brain-derived neurotrophic factor-, neurotrophin-3-, and tyrosine kinase receptor-like immunoreactivity in lingual taste bud fields of mature hamster after sensory denervation
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DOI：
10.1002/cne.2164
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发表时间：
2003-01-01
影响因子：
2.5
通讯作者：
Whitehead, MC
中科院分区：
文献类型：
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作者：
Ganchrow, D;Ganchrow, JR;Whitehead, MC
与大多数哺乳动物的舌味蕾不同，成熟仓鼠舌前部的菌状芽在感觉神经切断后仍能存活。神经营养因子配体、脑源性神经营养因子（BDNF）和神经营养因子-3（NT-3）以及它们各自的酪氨酸激酶（Trk）受体TrkB和TrkC在去神经支配的味蕾中的作用尚不清楚。本报告研究了胚细胞免疫反应性（IR）程度的变化（即，免疫反应细胞数/芽轮廓）和这些标记物在单侧去神经成熟仓鼠中的神经纤维-IR密度。将鼓索/舌神经切除后的菌状芽野与舌咽神经切除后的神经依赖性、舌后叶状和轮廓状芽野进行比较。损伤后4周，失神经的菌状芽的数量与未手术侧相匹配，而失神经的叶状和轮廓状芽的数量分别减少了72%和38%。在舌后部存活的味蕾中，与未手术侧相比，叶状芽细胞BDNF-、NT-3-和TrkB样IR以及轮廓芽细胞BDNF-和NT-3样IR的程度显著降低。相比之下，前舌菌状芽细胞，神经营养因子和受体样IR的程度相对较小的影响：NT-3和TrkB样IR不变，BDNF样IR，虽然显着下降，也保持。此外，TrkB样纤维IR基本上消除内和周围的菌状芽。因此，真菌状芽细胞中的NT-3、BDNF和TrkB样IR可能反映了促进生存的自分泌能力。因为芽细胞中不存在TrkC样IR（即，尽管NT-3在芽细胞TrkB中是免疫阴性的，并且在芽内和芽周的纤维中是稀疏的，但NT-3也可以结合芽细胞TrkB，从而在去神经支配后维持真菌状芽细胞活力。(C)2002 Wiley-Liss，Inc.
Unlike lingual taste buds in most mammals, fungiform buds on the anterior tongue of mature hamster survive sensory denervation. The role of the neurotrophin ligands, brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3), and their respective tyrosine kinase (Trk) receptors, TrkB and TrkC, in denervated taste buds is not known. The present report investigates changes in the degree of gemmal cell immunoreactivity (IR) (i.e., number of immunoreactive cells/bud profile) and density of nerve fiber-IR of these markers in unilaterally denervated mature hamsters. The fungiform bud field after chorda tympani/lingual nerve resection is compared with the nerve-dependent, posterior tongue foliate and circumvallate bud fields after glossopharyngeal nerve resection. Four weeks post lesion, the number of denervated fungiform buds matched that on the unoperated side, whereas denervated foliate and circumvallate bud counts decreased by 72% and 38%, respectively. In taste buds that survived on the posterior tongue, the degree of foliate bud cell BDNF-, NT-3-, and TrkB-like IR, and circumvallate bud cell BDNF- and NT-3-like IR, significantly decreased compared with the unoperated side. In contrast, for anterior tongue fungiform bud cells, the degree of neurotrophin- and receptor-like IR was relatively less affected: NT-3- and TrkB-like IR were unchanged; BDNF-like IR, although significantly decreased, was also maintained. Moreover, TrkB-like fiber IR was essentially eliminated within and surrounding fungiform buds. Hence, NT-3-, BDNF-, and TrkB-like IR in fungiform gemmal cells may reflect an autocrine capacity promoting survival. Because TrkC-like IR in bud cells is absent (i.e., immunonegative), and sparse in fibers intragemmally and perigemmally, NT-3 may also bind to bud cell TrkB so as to sustain fungiform gemmal cell viability post denervation. (C) 2002 Wiley-Liss, Inc.