VIRUS INTERFERENCE .2. SOME PROPERTIES OF INTERFERON
VIRUS INTERFERENCE .2. SOME PROPERTIES OF INTERFERON
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DOI：
10.1098/rspb.1957.0049
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发表时间：
1957-01-01
期刊：
影响因子：
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通讯作者：
VALENTINE, RC
中科院分区：
文献类型：
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作者：
ISAACS, A;LINDENMANN, J;VALENTINE, RC
干扰素可以通过在用甲型流感病毒攻击的绒毛尿囊膜片段中诱导的干扰量来滴定。在超过10倍的范围内，观察到干扰素浓度与攻毒病毒达到的血凝素滴度之间成反比。干扰素在2[degree]C下稳定2周。在60[degree]C下1 h后发生显著灭活。在100，000 g下1/2 h，未检测到干扰素沉淀。通过表观孔径为0.6 [mu]的gradocol过滤器将其截留。不可透析。干扰素对甲型流感病毒、仙台病毒、纽卡斯尔病毒和牛痘病毒有活性。它不被抗MEL兔血清中和，仅被富含甲型流感可溶性抗原补体结合抗体的合并人血清轻微抑制。
Interferon could be titrated by the amount of interference induced in fragments of chorio-allantoic membranes challenged with influenza A virus. Over a ten-fold range, inverse proportion between interferon concentration and haemagglutinin titre reached by the challenge virus was observed. Interferon proved stable at 2[degree]C for 2 weeks. Marked inactivation took place after 1 h at 60[degree]C. Interferon was not measurably sedimented by 100,000 g for 1/2 h. It was held back by gradocol filters of apparent pore diameter 0.6 [mu]. It was not dialyzable. Interferon was active against influenza A, Sendai, Newcastle disease and vaccinia viruses. It was not neutralized by anti-MEL rabbit serum and only slightly inhibited by pooled human serum rich in complement-fixing anti body to influenza A soluble antigen.