Downregulation of COX-2 and iNOS by amentoflavone and quercetin in A549 human lung adenocarcinoma cell line
Downregulation of COX-2 and iNOS by amentoflavone and quercetin in A549 human lung adenocarcinoma cell line
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DOI：
10.1054/plef.2002.0387
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发表时间：
2002-05-01
影响因子：
3
通讯作者：
Ziboh, VA
中科院分区：
文献类型：
--
作者：
Banerjee, T;Van der Vliet, A;Ziboh, VA
黄酮类化合物是广泛存在于植物界的天然多酚类化合物。据报道，它们对健康有许多有益的影响。在本研究中，我们利用槲皮素、双黄酮和黄烷酮，研究了不同的黄酮类化合物对细胞因子介导的环氧合酶-2和诱导型一氧化氮合酶在A549细胞中表达和活性的潜在影响。结果表明，50 mU浓度的槲皮素对A549细胞的PGE(2)生物合成有很强的抑制作用，而对COX-2mRNA和蛋白的表达几乎没有影响。与栎素不同的是，在另一组实验中，金刚烷黄酮强烈抑制了PGE(2)的生物合成和COX-2的mRNA和蛋白表达，完全抑制了iNOS蛋白的表达，而不影响iNOS的mRNA表达。与之相反，苦参素对iNOS m RNA表达无抑制作用，但对i NOS蛋白表达有微弱抑制作用，相同浓度的黄烷酮对上述两种酶均无抑制作用。综上所述，我们的研究结果表明，苦参素和栎素通过下调COX-2/iNOS的表达而不同程度地抑制PGE(2)的生物合成。(C)2002爱思唯尔科学有限公司。保留所有权利。
Flavonoids are natural polyphenolic compounds ubiquitously present in the plant kingdom. They are reported to exhibit numerous beneficial health effects. In the present study, we demonstrate the potential effects of different flavonolds on cytokines mediated cyclooxygenase-2 and inducible nitric oxide synthase expression and activities in A549 cell line using quercetin, amentoflavone and flavanone. Our data revealed that quercetin, at 50 muM concentration inhibited PGE(2) biosynthesis by A549 very strongly with little effect on COX-2 mRNA and protein expression. Unlike quercetin, amentoflavone inhibited both PGE(2) biosynthesis and COX-2 mRNA and protein expression strongly In another set of experiment, quercetin inhibited iNOS protein expression completely without affecting iNOS mRNA expression. In contrast, amentoflavone although exerted no inhibitory effect on iNOS m RNA expression, did inhibit weakly I NOS protein expression, Flavanone had no inhibitory effect on either enzyme at the same concentration. Taken together, our data indicated that amentoflavone and quercetin differentially exerted supression of PGE(2) biosynthesis via downregulation of COX-2/iNOS expression. (C) 2002 Elsevier science Ltd. All rights reserved.