The GCR1 gene of Saccharomyces cerevisiae is a split gene with an unusually long intron.
The GCR1 gene of Saccharomyces cerevisiae is a split gene with an unusually long intron.
复制标题
酿酒酵母的 GCR1 基因是一个分裂基因，具有异常长的内含子。
DOI：
10.1093/genetics/138.3.973
复制
发表时间：
1994
期刊：
影响因子：
3.3
通讯作者：
Santangelo,G
中科院分区：
文献类型：
--
作者：
Tornow,J;Santangelo,G
In Saccharomyces cerevisiae, GCRl encodes a transcrip tional activator that contacts at least two other proteins [Raplp and an unidentified component of the transcriptional machinery (TORNOW et al. 1993) l. Cells that lack Gcrlp grow much more slowly than wild-type cells and exhibit a reduction in transcription of glycolytic and translational component genes (CLI~ ON and FRAENKEL 1981; CLIFTON et al. 1978; SANTANGELO~~~ TORNOW 1990; TORNOW and SANTANGELO 1990). Activation of these genes is accomplished interdependently with Raplp (TORNOW et al. 1993), an essential multifunctional protein. Our laboratory is interested in determining the role of Gcrlp in transcriptional activation of its many target genes. During an analysis of the effects of point mutagenesis on GCRl, we discovered that it contains an intron, contrary to earlier published reports (HOLLAND et al. 1987; BAKER 1986). Given that this gene is of interest to many laboratories, we report here the evidence indicating the presence and location of the intron to correct the misconception that GCRl is not spliced.
