An In Vitro Blood-Feeding Method Revealed Differential Borrelia turicatae (Spirochaetales: Spirochaetaceae) Gene Expression After Spirochete Acquisition and Colonization in the Soft Tick Ornithodoros turicata (Acari: Argasidae)
An In Vitro Blood-Feeding Method Revealed Differential Borrelia turicatae (Spirochaetales: Spirochaetaceae) Gene Expression After Spirochete Acquisition and Colonization in the Soft Tick Ornithodoros turicata (Acari: Argasidae)
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DOI：
10.1093/jme/tjw171
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发表时间：
2017-02-01
影响因子：
2.1
通讯作者：
Marconi, Richard T.
中科院分区：
文献类型：
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作者：
Neelakanta, Girish;Sultana, Hameeda;Marconi, Richard T.
在美国中西部、西南部和南部，软蜱Ornithodoros turicata传播螺旋体Borrelia turiculata，这是人类回归热的病原体。在这项研究中，我们报告了一个简单而有效的体外饲养方法来评估O。图里卡塔湾turicillin相互作用若虫和成年雌蜱在体外取食B后均成功获得螺旋体。尿酸感染的血液。我们还注意到螺旋体对从喂食B的蜱虫蜕皮的成蜱的跨场传播。被尿酸感染的血液某些B的差异表达模式。在获得载体并定殖后，turiclactin基因是明显的。节肢动物相关的脂蛋白AlpmRNA的水平显着上调和H因子结合蛋白FhbA和免疫原性蛋白BipA的mRNA水平显着下调的螺旋体收购到蜱后，在培养基中生长的螺旋体相比。此外，在未进食蜱的螺旋体中，与获得后螺旋体中的水平相比，bta 124和bta 116等基因显著上调。这些发现代表了研究B的有效体外供血方法。在这些蜱中获得和定殖后的turiclactin基因表达。总之，我们报告说，B。当通过体外喂养获得时，蜱虫在蜱宿主中存活和发育。我们还报告说，B。与体外培养物相比，turiclactone基因在蜱中差异表达，表明蜱环境对螺旋体基因表达的影响。
In the Midwestern, Southwestern, and Southern part of the United States, the soft tick Ornithodoros turicata transmits the spirochete Borrelia turicatae, the causative agent of relapsing fever in humans. In this study, we report a simplified and an efficient method of in vitro feeding to evaluate O. turicata-B. turicatae interactions. Both nymphal and adult female ticks successfully acquired spirochetes upon in vitro feeding on the B. turicataeinfected blood. We also noted transstadial transmission of spirochetes to adult ticks that were molted from nymphs fed on B. turicatae-infected blood. A differential expression pattern for some of the B. turicatae genes was evident after acquisition and colonization of the vector. The levels of arthropod-associated lipoprotein AlpmRNA were significantly upregulated and the mRNA levels of factor H binding protein FhbA and immunogenic protein BipA were significantly downregulated in the spirochetes after acquisition into ticks in comparison with spirochetes grown in culture medium. In addition, genes such as bta124 and bta116 were significantly upregulated in spirochetes in unfed ticks in comparison with the levels noted in spirochetes after acquisition. These findings represent an efficient in vitro blood-feeding method to study B. turicatae gene expression after acquisition and colonization in these ticks. In summary, we report that B. turicatae survive and develop in the tick host when acquired by in vitro feeding. We also report that B. turicatae genes are differentially expressed in ticks in comparison with the in vitro-grown cultures, indicating influence of tick environment on spirochete gene expression.