DIFFERENCES IN CELL-TO-CELL SPREAD OF PATHOGENIC AND APATHOGENIC RABIES VIRUS INVIVO AND INVITRO
DIFFERENCES IN CELL-TO-CELL SPREAD OF PATHOGENIC AND APATHOGENIC RABIES VIRUS INVIVO AND INVITRO
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DOI：
10.1128/jvi.56.1.12-18.1985
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发表时间：
1985-01-01
影响因子：
5.4
通讯作者：
KOPROWSKI, H
中科院分区：
文献类型：
--
作者：
DIETZSCHOLD, B;WIKTOR, TJ;KOPROWSKI, H
致病性亲本狂犬病病毒和非致病性变异病毒在体内感染神经元和体外感染神经母细胞瘤细胞的能力不同。脑内接种后，两种病毒感染神经元在脑内的分布相似，但非致病原病毒在脑内扩散的速度、感染神经元的数量和细胞坏死程度要低得多。在小鼠神经母细胞瘤细胞上吸附后，非致病原病毒的内化速度慢于致病病毒，加入狂犬病病毒中和抗体可完全阻止无致病变异病毒在细胞间的传播，而致病病毒的传播不受影响。
Pathogenic parental rabies virus and apathogenic variant virus were shown to differ in their ability to infect neurons in vivo and neuroblastoma cells in vitro. After intracerebral inoculation, the distribution of infected neurons in the brain was similar for both viruses, but the rate of spread throughout the brain, the number of infected neurons, and the degree of cellular necrosis were much lower in the case of apathogenic virus. After adsorption to mouse neuroblastoma cells, apathogenic virus was less rapidly internalized than pathogenic virus, and cell-to-cell spread of apathogenic variant virus was completely prevented by the addition of rabies virus-neutralizing antibody, whereas the spread of pathogenic virus was not affected.