TYPE-X COLLAGEN-SYNTHESIS BY CULTURED CHONDROCYTES DERIVED FROM THE PERMANENT CARTILAGINOUS REGION OF CHICK-EMBRYO STERNUM
TYPE-X COLLAGEN-SYNTHESIS BY CULTURED CHONDROCYTES DERIVED FROM THE PERMANENT CARTILAGINOUS REGION OF CHICK-EMBRYO STERNUM
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DOI：
10.1016/0012-1606(87)90391-5
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发表时间：
1987-10-01
影响因子：
2.7
通讯作者：
CANCEDDA, R
中科院分区：
文献类型：
--
作者：
CASTAGNOLA, P;TORELLA, G;CANCEDDA, R
在发育中的鸡胚胸骨中，X型胶原由来自头侧区域（推定矿化区）的软骨细胞合成，而不是由来自尾侧区域（永久软骨区）的软骨细胞合成（Gibson et al., 1984， J. Cell Biol. 99, 208-216）。为了区分两种可能性，即永久软骨中存在不允许的微环境，或者尾侧软骨细胞本身无法成为肥大的x型细胞，我们从44期鸡胚胎胸骨尾侧三分之一分离出软骨细胞，并将其悬浮在琼脂糖包被的培养皿中培养。我们发现，在这些条件下，来自尾端区的软骨细胞分化为肥大软骨细胞，并合成大量的X型胶原，这是通过体外制备的标记蛋白的电泳模式和用特定cDNA探针对mrna进行槽点印迹分析所揭示的。
In the developing chick embryo sternum, type X collagen is synthesized by chondrocytes from the cephalic region (presumptive mineralization zone) but not by chondrocytes from the caudal region (permanent cartilaginous zone) (Gibson et al., 1984, J. Cell Biol. 99, 208-216). To distinguish between two possibilities, the presence of a nonpermissive microenvironment in the permanent cartilage or the intrinsic inability of caudal chondrocytes to become hypertrophic, type X-producing cells, we have isolated chondrocytes from the caudal third of stage 44 chick embryo sterna and grown them in suspension on agarose-coated dishes. We have found that in these conditions chondrocytes from the caudal zone differentiate to hypertrophic chondrocytes and synthesize large amount of type X collagen, as revealed by the electrophoretic pattern of labeled proteins made in vitro and by slot blot analysis of mRNAs with specific cDNA probes.