Effect of sevoflurane preconditioning on sleep reintegration after alteration by lipopolysaccharide
Effect of sevoflurane preconditioning on sleep reintegration after alteration by lipopolysaccharide
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DOI：
10.1111/jsr.13556
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发表时间：
2022-02
影响因子：
4.4
通讯作者：
Tsuyoshi Nemoto;Y. Irukayama-tomobe;Yuki Hirose;Hiromu Tanaka;Genki Takahashi;Satoshi Takahashi;Masashi Yanagisawa;T. Kanbayashi
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文献类型：
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作者：
Tsuyoshi Nemoto;Y. Irukayama-tomobe;Yuki Hirose;Hiromu Tanaka;Genki Takahashi;Satoshi Takahashi;Masashi Yanagisawa;T. Kanbayashi
尽管有大量证据表明七氟醚具有器官保护作用，但其对睡眠紊乱的影响仍不清楚。我们假设七氟醚预处理对全身性炎症引起的睡眠紊乱有积极影响。对C57BL/6J小鼠进行前瞻性、随机实验室调查。采用脂多糖（LPS）诱导的全身炎症小鼠模型研究七氟醚对睡眠恢复的影响。通过脑电图/肌电图（EEG/EMG）和组织学研究评估症状恢复情况。小鼠在腹腔注射LPS前后分别暴露于2%七氟醚。术后24 h记录脑电图和肌电图。在七氟醚/脂多糖处理后采集脑组织，并使用针对胆碱乙酰转移酶（ChAT）和Fos的个体抗体进行免疫染色。定量分析了桥脚被盖核和外侧背被盖核中ChAT阳性和ChAT/Fos双阳性细胞（PPTg/LDTg）。与对照组小鼠相比，经七氟醚预处理但未经后处理的小鼠在LPS刺激后的脑电图记录中显示快速眼动（REM）睡眠显著增加。他们还表现出较短的快速眼动潜伏期，表明从LPS改变的睡眠中早期恢复。七氟醚预处理保留了REM发作的发作次数。七氟醚预处理加LPS组的PPTg/LDTg中ChAT/Fos双阳性细胞多于LPS组。七氟醚预处理促进由全身性炎症引起的睡眠改变的恢复。PPTg/LDTg的激活被认为是睡眠重新整合的一种机制。这种恢复现象显示了在全身性炎症引起的睡眠障碍病例中的临床应用潜力。
Despite extensive evidence on the organ protective effects of sevoflurane, its effect on disturbed sleep remains unclear. We hypothesised that sevoflurane preconditioning positively impacts disturbed sleep caused by systemic inflammation. A prospective, randomised laboratory investigation was conducted in C57BL/6J mice. A mouse model of lipopolysaccharide (LPS)‐induced systemic inflammation was employed to investigate the effects of sevoflurane on sleep recovery. Symptom recovery was evaluated through electroencephalography/electromyography (EEG/EMG) and histological studies. The mice were exposed to 2% sevoflurane before and after peritoneal injection of LPS. The EEG and EMG were recorded for 24 h after the procedure. Brain tissue was harvested after the sevoflurane/LPS procedure and was immunostained using individual antibodies against choline acetyltransferase (ChAT) and Fos. The ChAT‐positive and ChAT/Fos double‐positive cells were analysed quantitatively in the pedunculopontine tegmental nucleus and laterodorsal tegmental nucleus (PPTg/LDTg). Compared with control mice, mice preconditioned with sevoflurane but not post‐conditioned showed a significant increase in rapid eye movement (REM) sleep during EEG recording following the LPS challenge. They also demonstrated a shorter REM latency, indicating an early recovery from LPS‐altered sleep. The bouts of REM episodes were retained with sevoflurane preconditioning. More ChAT/Fos double‐positive cells were observed in the PPTg/LDTg in the sevoflurane preconditioning plus LPS group than in the LPS‐only group. Sevoflurane preconditioning promotes recovery from altered sleep induced by systemic inflammation. Activation of PPTg/LDTg is considered a mechanism underlying sleep reintegration. The recovery phenomenon shows potential for clinical application in cases of sleep disturbances induced by systemic inflammation.