Iron hydroxamate transport of Escherichia coli: Nucleotide sequence of the fhuB gene and identification of the protein
Iron hydroxamate transport of Escherichia coli: Nucleotide sequence of the fhuB gene and identification of the protein
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大肠杆菌的异羟肟酸铁转运：fhuB 基因的核苷酸序列和蛋白质的鉴定
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
V. Braun
中科院分区：
文献类型：
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作者：
W. Köster;V. Braun
FhuA、fhuC和fhuD基因编码的产物参与了铁色素进入大肠杆菌细胞的运输。测定了与fhuD相邻的另一个以前命名为fhuB的基因座的核苷酸序列。它包含一个由659个氨基酸组成的多肽的开放阅读框架。含有fhuB区的质粒在体外转录/翻译系统中和在大肠杆菌微细胞中的表达表明，一种多肽在SDS-聚丙烯酰胺凝胶上具有极强的凝胶迁移率，这与所用的实验条件密切相关。这一性质可以用蛋白质的疏水性来解释，并得出结论：fhuB基因座由一个结构基因组成，编码一个相对分子质量为82,181的膜蛋白。FhuB基因是所有通过异羟甲酸酯化合物运行的铁运输系统所必需的。Fhu操纵子中基因的顺序是fhuA fhuC fhuD fhuB，转录从fhuA到fhuB进行。
SummaryThe genes fhuA, fhuC and fhuD encode products involved in the transport of ferrichrome into cells of Escherichia coli. The nucleotide sequence of an additional locus contiguous to fhuD and formerly designated fhuB was determined. It contains an open reading frame for a polypeptide that consists of 659 amino acids. Expression of plasmids containing the fhuB region in an in vitro transcription/translation system and in E. coli minicells revealed a polypeptide with an electrophoretic mobility on SDS-polyacrylamide gels extremely dependent on the experimental conditions employed. This property could be explained by the very hydrophobic nature of the protein and it was concluded that the fhuB locus consists of a structural gene that encodes a membrane protein with a molecular weight of 82,181. The fhuB gene is required for all iron transport systems which operate via hydroxamate compounds. The order of the genes in the fhu operon is fhuA fhuC fhuD fhuB, and transcription proceeds from fhuA to fhuB.