A NEW VERTICAL MESH TRANSFER TECHNIQUE FOR METAL-TOLERANCE STUDIES IN ARABIDOPSIS - ECOTYPIC VARIATION AND COPPER-SENSITIVE MUTANTS
A NEW VERTICAL MESH TRANSFER TECHNIQUE FOR METAL-TOLERANCE STUDIES IN ARABIDOPSIS - ECOTYPIC VARIATION AND COPPER-SENSITIVE MUTANTS
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DOI：
10.1104/pp.108.1.29
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发表时间：
1995-05-01
期刊：
影响因子：
7.4
通讯作者：
TAIZ, L
中科院分区：
文献类型：
--
作者：
MURPHY, A;TAIZ, L
为了快速分离植物耐金属突变体，研究开发了一种新的垂直网格转移（VMT）技术。该技术是定量的，可以比较不同菌株或生态型的生长反应。利用VMT技术，研究了10个生态型拟南芥对Cu2+、Zn2+、Ni2+、Cr3+、Cd2+和Al3+的剂量反应。观察到6种金属在最高浓度无抑制和最低浓度完全抑制的生态型差异。两个生态型w和Enkheim对铜表现出诱导耐受机制。对铜没有抑制作用的最高浓度的Ws的预处理导致最低浓度的转移，使铜的完全抑制作用向更高的值转移。Cu2+和Zn2+表现出部分交叉诱导和交叉耐受。此外，使用VMT程序筛选甲基磺酸乙酯诱变哥伦比亚种子中的铜敏感（cus）突变体。到目前为止，59个假定的cos突变体在M(4)或M(5)代的重新测试中幸存下来。当在添加了30 μ M CuCl2的结冷胶上生长时，cus突变体表现出明显的毒性症状。cus1突变体的铜剂量响应曲线显示，金属敏感表型对较低浓度范围具有特异性。
A new vertical mesh transfer (VMT) technique has been developed to facilitate the rapid isolation of plant metal-tolerance mutants. The technique is quantitative, allowing comparisons of the growth responses of different strains or ecotypes. Using the VMT technique, we have characterized the dose responses of 10 ecotypes of Arabidopsis thaliana to Cu2+, Zn2+, Ni2+, Cr3+, Cd2+, and Al3+. Ecotypic variations in the highest concentration causing no inhibition and the lowest concentration causing complete inhibition for the six metals were observed. Two ecotypes, Ws and Enkheim, exhibited an inducible tolerance mechanism in response to copper. Pretreatment of Ws with the highest concentration causing no inhibition for copper resulted in a shifting of the lowest concentration causing complete inhibition to a higher value. Partial cross-induction and cross-tolerance between Cu2+ and Zn2+ were demonstrated. In addition, ethyl methanesulfonate-mutagenized Columbia seeds were screened for copper-sensitive (cus) mutants using the VMT procedure. Thus far, 59 putative cos mutants have survived retesting to the M(4) or M(5) generation. When grown on gellan gum supplemented with 30 mu M CuCl2, cus mutants develop marked toxicity symptoms. A copper dose-response curve of the cus1 mutant showed that the metal-sensitive phenotype is specific for the lower concentration range.