Incidence of Citrus vein enation virus in Citrus spp. and Poncirus trifoliata in Korea.
Incidence of Citrus vein enation virus in Citrus spp. and Poncirus trifoliata in Korea.
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DOI：
10.5423/rpd.2019.25.4.233
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发表时间：
2019-01-01
影响因子：
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通讯作者：
Lee, S. J.
中科院分区：
文献类型：
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作者：
Kim, Bong-Sub;Yang, Hee-Ji;Lee, S. J.
柑橘脉突病毒（CVEV）在韩国被规定为检疫病毒，于2017年首次在济州岛发现。2018年2月，进行了一项调查，以确定CVEV在种植柑橘的主要商业区的分布。和枳。该调查在朝鲜南部半岛和济州岛的203个格罗夫斯进行。采用逆转录聚合酶链反应检测和序列测定法确定CVEV感染。从CVEV感染的样品中获得的外壳蛋白（CP）基因序列与先前报道的CVEV CP序列具有较高的相似性（超过98%）。结果表明，在136个格罗夫斯（67%）中检测到CVEV，其中香橙和温州蜜柑的感染率分别为85.4%和77.8%。济州岛CVEV感染率较高（90.6%）。我们的研究结果表明，CVEV已在韩国柑橘和枳壳中广泛传播。根据该结果，韩国检疫机构决定将CVEV从韩国检疫中排除。
Citrus vein enation virus (CVEV), which was regulated as a quarantine virus in Korea, was firstly found on Jeju Island in 2017. In February 2018, a survey was carried out to determine the distribution of CVEV in the main commercial areas growing Citrus spp. and Poncirus trifoliata. The survey was performed at 203 groves in the southern Korean Peninsula and Jeju Island. CVEV infection was determined by reverse transcription polymerase chain reaction detection and sequencing. The coat protein (CP) gene sequences obtained from the CVEV-infected samples showed high similarities (more than 98%) to the previously reported CVEV CP sequences. In summary, CVEV was detected in 136 groves (67%), in which 85.4% of Citrus junos and 77.8% of Citrus unshiu were infected by CVEV. In Jeju Island, the infection rate of CVEV was relatively higher (90.6%). Our result revealed that CVEV has spread widely in Citrus and Poncirus in Korea. Based on the result, the Korean quarantine agency decide to exclude CVEV from quarantine in Korea.