Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil
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DOI：
10.1590/s0034-89102007000300010
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发表时间：
2007-06-01
期刊：
影响因子：
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通讯作者：
Ito, Fumio Honma
中科院分区：
文献类型：
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作者：
Scheffer, Karin Corrêa;Carrieri, Maria Luiza;Ito, Fumio Honma
目的：鉴定参与维持狂犬病周期的蝙蝠种类；目的研究狂犬病毒在蝙蝠组织器官中的分布及接种小鼠的死亡时间。方法：2002年4月至2003年11月，采用直接免疫荧光法对来自圣保罗州各市的蝙蝠进行狂犬病毒筛查。通过用不同器官和组织以及大脑和唾液腺的碎片制备的20%悬浮液接种小鼠和N2A细胞来评估病毒在蝙蝠中的分布。在脑内接种后，每天监测小鼠的死亡时间。结果：在接收的4,395只蝙蝠中，发现1.9%的狂犬病病毒阳性。分属10属，以食虫为主。吸血蝙蝠、食虫蝙蝠、食果蝙蝠分别分别为15.33 +/- 2.08天和11.33 +/- 2.30天和16.45 +/- 4.48天和18.91 +/- 6.12天和12.60 +/- 2.13天和15.67 +/- 4.82天和12.60 +/- 2.13天和15.67 +/- 4.82天。结论：感染狂犬病毒的种类为：斜纹Artibeus lituratus、Artibeus sp.、nigricans Myotis、Myotis sp.、Eptesicus sp.、Lasiurus egereus、Nyctinomops laticaudatus、Tadarida brasiliensis、tetiotus velatus、Molossus rufus、Eumops sp.和desmotus rotundus。不同组织器官的病毒调查表明，大脑和唾液腺是最适合分离病毒的部位。
OBJECTIVE: To identify the species of bats involved in maintaining the rabies cycle; to investigate the distribution of the rabies virus in the tissues and organs of bats and the time taken for mortality among inoculated mice.METHODS: From April 2002 to November 2003, bats from municipalities in the State of Sao Paulo were screened for the presence of the rabies virus, by means of direct immunofluorescence. The virus distribution in the bats was evaluated by inoculating mice and N2A cells with 20% suspensions prepared from fragments of different organs and tissues, plus the brain and salivary glands. The time taken for mortality among the mice was monitored daily, following intracerebral inoculation.RESULTS: Out of the 4,395 bats received, 1.9% were found positive for the rabies virus. They belonged to ten genera, with predominance of insectivores. The maximum mean times taken for mortality among the mice following inoculation with brain and salivary gland material were 15.33 +/- 2.08 days and 11.33 +/- 2.30 days for vampire bats, 16.45 +/- 4.48 days and 18.91 +/- 6.12 days for insectivorous bats, and 12.60 +/- 2.13 days and 15.67 +/- 4.82 days for frugivorous bats, respectively.CONCLUSIONS: The species infected with the rabies virus were: Artibeus lituratus, Artibeus sp., Myotis nigricans, Myotis sp., Eptesicus sp., Lasiurus ega, Lasiurus cinereus, Nyctinomops laticaudatus, Tadarida brasiliensis, Histiotus velatus, Molossus rufus, Eumops sp. and Desmodus rotundus. Virus investigation in the different tissues and organs showed that the brain and salivary glands were the most suitable sites for virus isolation.