Quantification of myocardial injury produced by temporary coronary artery occlusion and reflow with technetium-99m-pyrophosphate.
Quantification of myocardial injury produced by temporary coronary artery occlusion and reflow with technetium-99m-pyrophosphate.
复制标题
使用锝-99m-焦磷酸对临时冠状动脉闭塞和回流产生的心肌损伤进行量化。
DOI：
10.1161/01.cir.75.3.611
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发表时间：
1987
期刊：
影响因子：
37.8
通讯作者：
Willerson,JT
中科院分区：
文献类型：
--
作者：
Jansen,DE;Corbett,JR;Buja,LM;Hansen,C;Ugolini,V;Parkey,RW;Willerson,JT
此前，锝-99m-焦磷酸亚锡（99mTc-PPi）已被用于动物永久性冠状动脉闭塞后心肌梗死的定位和估计。本研究验证了99mTc-PPi对狗暂时性冠状动脉闭塞和再流引起的心肌梗死的准确大小的假设。实验分为三组：A组闭塞3小时，再灌注2小时，回流后10分钟注射99mTc-PPi (n = 10)；B组闭塞3小时，再灌注2小时，回流后90分钟注射99mTc-PPi (n = 11)；C组闭塞3小时，血流灌注后10分钟注射99mTc-PPi，血流灌注后48小时再次注射99mTc-PPi （n = 5）。对A、B组狗心肌切片进行体外成像。C组犬在体内进行单光子发射计算机断层扫描（SPECT），并在研究结束时进行心肌切片成像。心肌梗死范围用三苯基四唑氯（TTC）染色确定，冠状动脉血流量用放射性微球估计。此外，从心肌梗死中心、心肌梗死外侧部分、与梗死外侧相邻的正常心肌和正常心肌取跨壁心肌组织样本，计数99mTc-PPi活性。在A组（r = 0.89）和B组（r = 0.98）中，由99mTc-PPi摄取增加的区域确定的梗死面积与TTC染色估计的梗死面积之间存在显著相关性。（摘要删节250字）
Previously, technetium-99m-stannous pyrophosphate (99mTc-PPi) has been used to localize and estimate the size of myocardial infarcts in animals after permanent coronary artery occlusion. This study tested the hypothesis that 99mTc-PPi accurately sizes myocardial infarctions produced by temporary coronary artery occlusion and reflow in dogs. Three groups of dogs were studied: group A underwent 3 hr of occlusion followed by 2 hr of reperfusion, with 99mTc-PPi injected 10 min after reflow (n = 10); group B underwent 3 hr of occlusion followed by 2 hr of reperfusion, with 99mTc-PPi injected 90 min after reflow (n = 11); and group C underwent 3 hr of occlusion followed by reflow with 99mTc-PPi injected at 10 min and again at 48 hr after reflow (n = 5). Myocardial slices from group A and B dogs were imaged in vitro. Group C dogs were imaged with single photon-emission computed tomography (SPECT) in vivo, and myocardial slices were imaged in vitro at the conclusion of the study. The extent of myocardial infarction was defined with triphenyltetrazolium chloride (TTC) staining, and coronary blood flow was estimated with radioactive microspheres. In addition, transmural myocardial tissue samples were taken from the center of the myocardial infarction, the lateral portion of the myocardial infarction, the normal myocardium adjacent to the lateral aspect of the infarcts, and from the normal myocardium and counted for 99mTc-PPi activity. A significant correlation was found between infarct size determined by areas of increased 99mTc-PPi uptake and that estimated from TTC staining for both group A (r = .89) and group B animals (r = .98).(ABSTRACT TRUNCATED AT 250 WORDS)