SULFONOLIPID CONTENT OF CYTOPHAGA AND FLEXIBACTER SPECIES ISOLATED FROM SOIL AND CULTURED UNDER DIFFERENT NUTRIENT AND TEMPERATURE REGIMES
SULFONOLIPID CONTENT OF CYTOPHAGA AND FLEXIBACTER SPECIES ISOLATED FROM SOIL AND CULTURED UNDER DIFFERENT NUTRIENT AND TEMPERATURE REGIMES
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DOI：
10.1139/m94-021
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发表时间：
1994-02-01
影响因子：
2.8
通讯作者：
MCGILL, WB
中科院分区：
文献类型：
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作者：
DRIJBER, RA;MCGILL, WB
嗜纤维菌属和嗜纤维菌属的滑行细菌在其外膜中含有一种不寻常的磺脂，这可能是土壤中这些生物的潜在生化标志物。一个标记提供生物量信息的精确度随所研究的分类单元细胞内标记含量的范围而变化。为了评估这一点，噬纤维菌属（Cytophaga）和弯曲杆菌属（Flexibacter spp.）从粪便颗粒和土壤中分离，用[S-35]硫酸盐分批培养，并提取脂质。采用同位素稀释技术测定磺酰脂质S，采用比色法测定脂质磷酸盐。在各种条件下生长的分离株之间的磺酰脂质S的蛋白质和磷脂P的比率进行了比较。磺脂S与磷脂P的比率为0.22至0.98，证实磺脂是噬纤维菌属和嗜纤维菌属的主要细胞组分。满足了磺脂类作为土壤中这些生物的生物标志物的成功所必需的两个标准：（i）非纤维素分解分离株中的磺脂类浓度，除了一个例外，跨越一个狭窄的范围（40-60 μ mol S/g细胞蛋白），和（ii）在这里研究的生长条件下，保持磺脂类浓度的这个范围。然而，纤维素分解噬细胞菌可以清楚地与其他噬纤维菌属和嗜纤维杆菌属区分开来。以磺脂含量计，85-164 μ mol S/g细胞蛋白。在解释将磺脂类用作噬纤维菌属和嗜纤维菌属定量生物标志物的数据时，必须考虑纤维素分解分离株和非纤维素分解分离株之间磺脂类含量的二分法。在纤维素分解噬细胞菌占优势的栖息地。
Gliding bacteria of the genera Cytophaga and Flexibacter contain an unusual sulfonolipid in their outer membrane that may be a potential biochemical marker for these organisms in soil. The precision with which a marker provides information about biomass varies with the range in marker content within the cells of the taxon under study. To evaluate this, Cytophaga and Flesibacter spp. were isolated from earthworm fecal pellets and soil, batch cultured with [S-35]sulfate, and extracted for lipids. Sulfonolipid S was measured using isotope dilution techniques and Lipid phosphate was determined by colorimetry. Ratios of sulfonolipid S to protein and to phospholipid P were compared among isolates grown under various conditions. Ratios of sulfonolipid S to phospholipid P from 0.22 to 0.98 confirmed sulfonolipids as major cell components of Cytophaga and Flexibacter spp. Two criteria essential to the success of sulfonolipids as a biomarker for these organisms in soil were met: (i) sulfonolipid concentration among the noncellulolytic isolates, with one exception, spanned a narrow range (40-60 mu mol S/g cell protein), and (ii) this range in sulfonolipid concentration was maintained under the range of growth conditions studied here. Cellulolytic cytophagas, however, could be clearly differentiated from other Cytophaga and Flexibacter spp. on the basis of sulfonolipid content, 85-164 mu mol S/g cell protein. This dichotomy in sulfonolipid content between cellulolytic and noncellulolytic isolates must be considered when interpreting data where sulfonolipids are used as a quantitative biomarker for Cytophaga and Flexibacter spp. in habitats where cellulolytic cytophagas are dominant.