Bone marrow mesenchymal stem cells modified by angiogenin-1 promotes tissue repair in mice with oxygen-induced retinopathy of prematurity by promoting retinal stem cell proliferation and differentiation
Bone marrow mesenchymal stem cells modified by angiogenin-1 promotes tissue repair in mice with oxygen-induced retinopathy of prematurity by promoting retinal stem cell proliferation and differentiation
复制标题
血管生成素-1修饰的骨髓间充质干细胞通过促进视网膜干细胞增殖和分化促进氧诱导早产儿视网膜病变小鼠的组织修复
DOI：
10.1002/jcp.28706
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发表时间：
2019-11-01
影响因子：
5.6
通讯作者：
Feng，Zhi-Chun
中科院分区：
文献类型：
--
作者：
Ma，Qian-Qian;Liu，Fang-Yu;Feng，Zhi-Chun
视网膜病变已成为世界范围内导致失明的主要因素之一。虽然许多临床治疗方法都关注此类疾病，但大多数都侧重于缓解症状。本研究旨在探讨视网膜干细胞(RSCs)与血管生成素-1基因修饰的骨髓间充质干细胞(Ang-1-BMSCs)共培养对氧源性早产儿视网膜病变(OIR-ROP)小鼠视网膜组织损伤的影响。建立OIR-ROP小鼠模型后，将Ang-1-BMSCs、RSCs和OIR-ROP视网膜组织在Transwell小室中共培养。观察RSC的增殖情况、培养上清液中血管生成素-1、胰岛素样生长因子-1的表达以及β-微管蛋白和蛋白激酶C的表达。最后通过注射Ang-1-BMSCs + RSCs观察OIR-ROP小鼠视网膜组织的修复情况。在OIR-ROP小鼠模型中，与OIR-ROP视网膜组织共培养的RSC可被诱导分化为表达β-微管蛋白和蛋白激酶C的细胞，并促进 + -1和IGF1的表达。综上所述，本研究为Ang-1-BMSCs与RSCs共培养能促进RSCs的增殖和分化，改善OIR-ROP小鼠受损视网膜组织的治疗提供了证据。
Retinopathy has become one of the major factors that lead to blindness worldwide. Although many clinical therapies are concerned about such disease, most of them focus on symptoms alleviation. In this study, we aim to investigate whether coculture retinal stem cells (RSCs) with bone marrow mesenchymal stem cells transfected with angiogenin‐1 (Ang‐1‐BMSCs) affects the damaged retinal tissue of oxygen‐induced retinopathy of prematurity (OIR‐ROP) mice. After OIR‐ROP mouse model establishment, Ang‐1‐BMSCs, RSCs, and OIR‐ROP retinal tissues were cocultured in a a transwell chamber. RSCs proliferation and the expression of Ang‐1, insulin‐like growth factor‐1 (IGF‐1) in the supernatant of RSCs, as well as β‐tubulin and protein kinase C (PKC) expression were evaluated. Finally, the repair of OIR‐ROP mice retinal tissues was observed by injecting Ang‐1‐BMSCs + RSCs. In the OIR‐ROP mouse model, RSCs cocultured with OIR‐ROP retinal tissues could be induced to differentiate into cells expressing β‐tubulin and PKC and promote the expression of Ang‐1 and IGF‐1. coculture of Ang‐1‐BMSCs further enhanced the proliferation and differentiation of RSCs by promoting the expression of Ang‐1 and IGF‐1. Coculture of RSCs + Ang‐1‐BMSCs induced differentiation of Ang‐1‐BMSCs through interaction among intercellular factors and restored the damaged retinal tissue of OIR‐ROP mice. Collectively, our study provided evidence that coculture of Ang‐1‐BMSCs and RSCs could promote the proliferation and differentiation of RSCs and improve the treatment for the damaged retina tissue of OIR‐ROP mice.