Drug effects on the electrochemical detection of norepinephrine with carbon fiber and diamond microelectrodes
Drug effects on the electrochemical detection of norepinephrine with carbon fiber and diamond microelectrodes
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DOI：
10.1016/j.jelechem.2009.03.022
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发表时间：
2009-07-01
影响因子：
4.5
通讯作者：
Swain, Greg M.
中科院分区：
文献类型：
--
作者：
Dong, Hua;Wang, Shihua;Swain, Greg M.
去甲肾上腺素 (NE) 是一种由交感神经释放的血管收缩神经递质，支配动脉和静脉的平滑肌细胞。它是血管张力的重要调节剂。该分子具有电活性，可以使用碳纤维或掺硼金刚石微电极使用伏安法或电流法在血管表面进行局部测量。几种神经药理学药物通常用于去甲肾上腺素能神经效应物传递的研究。对于电化学方法，重要的是要了解 (i) 特定药物在用于检测 NE 的电位下是否具有电活性，和/或 (ii) 微电极暴露于药物是否会因药物吸附和/或结垢而导致 NE 氧化电流衰减。我们在此报告了对几种常用于研究交感神经效应器传递的药物的电化学活性的循环伏安研究，以及暴露于药物如何影响 NE 的电极响应灵敏度。比较了碳纤维和金刚石微电极的性能。结果表明，在用于检测 NE 的电位下，可卡因、咪唑克生和 PPADS（吡哆醛-磷酸-6-偶氮苯基-2',4'-二磺酸盐）在两个微电极上均没有电化学活性，并且药物暴露对 NE 氧化电流几乎没有影响。另一方面，接触辣椒素、哌唑嗪、育亨宾和 UK 14,304 会在两种微电极类型上产生不同程度的 NE 氧化电流衰减。此外，在哌唑嗪或辣椒素存在的情况下检测 NE 变得复杂，因为这两种药物在接近用于 NE 监测的电位时都会被氧化。当育亨宾和 UK 14,304 存在时，可以检测到 NE，但氧化电流降低。结果表明，在使用碳纤维或金刚石微电极进行体外或体内电化学测量之前，应确定任何药物对电极响应灵敏度和稳定性的影响。 (C) 2009 年，Elsevier B.V. 出版
Norepinephrine (NE) is a vasoconstricting neurotransmitter released from the sympathetic nerves that innervate the smooth muscle cells of arteries and veins. It is an important regulator of vascular tone. The molecule is electroactive and can be measured locally at the blood vessel surface using voltammetric or amperometric methods with both carbon fiber or boron-doped diamond microelectrodes. Several neuropharmacological agents are routinely employed in the study of noradrenergic neuroeffector transmission. With electrochemical methods, it is important to know (i) if a particular drug is electroactive at the potentials used to detect NE and or (ii) if exposure of the microelectrode to the drug causes any attenuation of the NE oxidation current because of drug adsorption and or fouling. We report herein on a cyclic voltammetric investigation of the electrochemical activity of several drugs commonly used to study sympathetic neuroeffector transmission, and how exposure to the drugs affects the electrode response sensitivity for NE. The performance of carbon fiber and diamond microelectrodes was compared. Results revealed that cocaine, idazoxan and PPADS (pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate) are electrochemically inactive at both microelectrodes at the potentials used to detect NE, and drug exposure has little effect on the NE oxidation current. On the other hand, exposure to capsaicin, prazosin, yohimbine and UK 14,304 produced varying degrees of NE oxidation current attenuation at both microelectrode types. Furthermore, detection of NE in the presence of prazosin or capsaicin is complicated by the fact that both drugs are oxidized near the potentials used for NE monitoring. When yohimbine and UK 14,304 are present, NE can be detected but the oxidation current is reduced. The results demonstrate that the effect of any drug on the electrode response sensitivity and stability should be ascertained before making in vitro or in vivo electrochemical measurements using carbon fiber or diamond microelectrodes. (C) 2009 Published by Elsevier B.V.