Effects of iron oxide incorporation for long term cell tracking on MSC differentiation in vitro and in vivo
Effects of iron oxide incorporation for long term cell tracking on MSC differentiation in vitro and in vivo
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DOI：
10.1016/j.bbrc.2008.02.159
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发表时间：
2008-05-16
影响因子：
3.1
通讯作者：
Bernsen, Monique
中科院分区：
文献类型：
--
作者：
Farrell, Eric;Wielopolski, Piotr;Bernsen, Monique
成功的细胞治疗将取决于监测移植细胞的能力。对于细胞标记，重要的是要证明有效的长期标记而不会对细胞表型和分化能力产生有害影响。我们证明了间充质干细胞 (MSC) 在体内可长期（7 周）保留超顺磁性氧化铁颗粒 (SPIO)，并可通过 MRI 检测到。在体外，通过 SPIO 标记和未标记细胞的组织学评估和分子分析证明了多谱系分化（成骨、软骨形成和脂肪形成）。在脂肪形成和软骨形成条件下，基因表达水平与未标记的对照相当，但在成骨条件下则不然。 MSCs 接种到支架中 21 天并皮下植入裸鼠体内 4 周，与植入的未标记对照支架相比，SPIO 标记的样品中显示出显着改变的表型，表明软骨形成分化。这项研究证明了使用 SPIO 和 MRI 的长期 MSC 可追溯性，SPIO 标记后不受抑制的多谱系 MSC 分化，尽管有微妙但显着的表型改变。 (C) 2008 Elsevier Inc. 保留所有权利。
Successful cell therapy will depend on the ability to monitor transplanted cells. With cell labeling, it is important to demonstrate efficient long term labeling without deleterious effects on cell phenotype and differentiation capacity. We demonstrate long term (7 weeks) retention of superparamagnetic iron oxide particles (SPIO) by mesenchymal stem cells (MSCs) in vivo, detectable by MRI. In vitro, multilineage differentiation (osteogenic, chondrogenic and adipogenic) was demonstrated by histological evaluation and molecular analysis in SPIO labeled and unlabeled cells. Gene expression levels were comaparable to unlabeled controls in adipogenic and chondrogenic conditions however not in the osteogenic condition. MSCs seeded into a scaffold for 21 days and implanted subcutaneously into nude mice for 4 weeks, showed profoundly altered phenotypes in SPIO labeled samples compared to implanted unlabeled control scaffolds, indicating chondrogenic differentiation. This study demonstrates long term MSC traceability using SPIO and MRI, uninhibited multilineage MSC differentiation following SPIO labeling, though with subtle but significant phenotypical alterations. (C) 2008 Elsevier Inc. All rights reserved.