Incunabular immunological events in prion trafficking.
Incunabular immunological events in prion trafficking.
复制标题
朊病毒贩运中的不期免疫事件。
DOI：
10.1038/srep00440
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发表时间：
2012
影响因子：
4.6
通讯作者：
Zabel,MarkD
中科院分区：
文献类型：
--
作者：
Michel,Brady;Meyerett-Reid,Crystal;Johnson,Theodore;Ferguson,Adam;Wyckoff,Christy;Pulford,Bruce;Bender,Heather;Avery,Anne;Telling,Glenn;Dow,Steven;Zabel,MarkD
虽然朊病毒可能在感染后立即与先天免疫系统相互作用，但对这种最初的对抗知之甚少。在这里，我们通过跟踪高富集的荧光朊病毒从感染部位到引流淋巴结，研究了嗜淋巴性和结内朊病毒运输的不可见事件。我们检测到外周朊病毒接种后，朊病毒从初始进入部位呈双相淋巴性转运。在给药后两小时内，朊病毒自动到达引流淋巴结细胞。单核细胞和树突状细胞（dc）需要补体，以在第二波朊病毒运输数小时后将最佳朊病毒运送到淋巴结。6小时时，B细胞占纵隔淋巴结中携带朊病毒细胞的大多数，表明结内朊病毒接受来自常驻dc或囊下窦巨噬细胞或直接来自滤泡导管。这些数据揭示了新的、细胞自主的朊病毒嗜淋巴性和B细胞在结内朊病毒运动中的突出作用。
While prions probably interact with the innate immune system immediately following infection, little is known about this initial confrontation. Here we investigated incunabular events in lymphotropic and intranodal prion trafficking by following highly enriched, fluorescent prions from infection sites to draining lymph nodes. We detected biphasic lymphotropic transport of prions from the initial entry site upon peripheral prion inoculation. Prions arrived in draining lymph nodes cell autonomously within two hours of intraperitoneal administration. Monocytes and dendritic cells (DCs) required Complement for optimal prion delivery to lymph nodes hours later in a second wave of prion trafficking. B cells constituted the majority of prion-bearing cells in the mediastinal lymph node by six hours, indicating intranodal prion reception from resident DCs or subcapsulary sinus macrophages or directly from follicular conduits. These data reveal novel, cell autonomous prion lymphotropism and a prominent role for B cells in intranodal prion movement.