Operational Laboratory Methods for GDGTs Groups Separation
Operational Laboratory Methods for GDGTs Groups Separation
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GDGT 组分离的操作实验室方法
DOI：
10.1007/s11802-020-4339-y
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发表时间：
2020
影响因子：
1.6
通讯作者：
Jia Guodong
中科院分区：
文献类型：
--
作者：
Liu Zhan;Li Li;He Juan;Chen Lingdi;Wang Junjian;Jia Guodong
甘油二烷基甘油四醚(GDGTS)是一种主要由细菌和古菌合成的特异性膜脂，可分为类异戊二烯和甲基支链烷基GDGTS(iGDGTS和brGDGTS)。本研究采用半制备高效液相色谱和硅胶柱层析的方法分离和收集了泥炭样品中的三个GDGTS基团(iGDGTS、brGDGTS和其他膜脂)。采用半制备高效液相色谱法和凝胶柱层析法，整个分析过程的回收率分别为85%−55%和70%−20%。此外，在每种方法中，brGDGts和iGDGts的回收率相似，尽管它们的含量有很大差异。根据乙醚裂解和还原测定，在分级基团中发现了高纯度。此外，半制备高效液相色谱法可以实现比硅胶法更好的分离效率，但耗时长，需要昂贵的设备，而硅胶层析法具有节省时间和操作方便的优点，但分离效率略有下降。硅胶法的优点使其成为批量实验和同位素研究的可操作的实验室方法。
Glycerol dialkyl glycerol tetraethers (GDGTs), specific membrane lipids synthesized mainly by bacteria and archaea, can be divided into isoprenoids and methyl branched alkyl GDGTs (iGDGTs and brGDGTs, respectively). Three GDGTs groups (iGDGTs, brGDGTs, and other membrane lipids) in a peat sample were separated and collected in this study using semi-preparative high-performance liquid chromatography (HPLC) and silica gel column chromatography. The obtained recoveries for the whole analytical procedure were 85%−55% using semi-preparative HPLC and 70%−20% using gel column chromatography. In addition, in each method, the recoveries of brGDGTs and iGDGTs were similar, regardless of the huge difference in their contents. High purity was found in the fractionated groups, determined based on ether cleavage and reduction. Moreover, the semi-preparative HPLC method could realize a better separation efficiency than the silica gel method, but it was time-consuming and required expensive equipment, while the silica gel chromatography method featured merits of time saving and convenient operation at the cost of a slight reduction in separation efficiency. The advantages of the silica gel method make it an operational laboratory method for batch experiments and isotopic studies.