Microbial changes after full-mouth tooth extraction, followed by 2-stage implant placement
Microbial changes after full-mouth tooth extraction, followed by 2-stage implant placement
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DOI：
10.1111/j.1600-051x.2011.01728.x
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发表时间：
2011-06-01
影响因子：
6.7
通讯作者：
Van Assche, Nele
中科院分区：
文献类型：
--
作者：
Quirynen, Marc;Van Assche, Nele
近年来的研究表明，定量聚合酶链式反应可以在全口拔牙后低浓度地检测出与牙周炎和种植周炎相关的细菌。这项研究监测了拔牙后9个月以上，基牙连接后1年的微生物区系。材料和方法10例重度牙周炎患者。拔牙六个月后，植入种植体。三到六个月后，它们连接到基牙上。从拔牙前至基牙连接后1年内，从舌背、唾液和龈下(牙齿/种植体)采集菌斑样本，并通过培养、定量聚合酶链式反应和棋盘技术进行分析。唾液和舌背上的牙龈卟啉单胞菌和连翘坦纳氏菌的浓度(qPCR和棋盘格)有所降低。中间普氏杆菌的变化可以忽略不计，伴生放线杆菌的变化也不明显。原始的龈下壁龛很快就被关键病原体侵占了。随着时间的推移，它们的最终浓度仍然很低，而检测频率仍然很高。结论完全拔牙可以显著减少与牙周炎和种植周炎相关的细菌，但伴生放线菌除外，这可能表明关键病原体可以在没有口袋的情况下存活。
BackgroundRecent studies showed that qPCR could detect bacteria related to periodontitis and peri-implantitis in a low concentration after full-mouth tooth extraction. This study monitored the microbiota from tooth extraction, over 9 months of full edentulism, up to 1 year after abutment connection.Material and methodsTen patients with severe periodontitis were recruited. Six months after tooth extraction, implants were inserted. Three to 6 months later, they were connected to abutments. Plaque samples were collected from the tongue dorsum, saliva, and subgingival area (teeth/implants) before extraction up to 1 year after abutment connection, and analysed via culture, qPCR, and checkerboard technology.ResultsA reduction in the total amount of aerobic and anaerobic CFU/ml was observed. The concentration of Porphyromonas gingivalis and Tannerella forsythia (qPCR and checkerboard) in the saliva and, to a lower extent, on the tongue dorsum reduced. For Prevotella intermedia, changes were negligible and no changes could be detected for Aggregatibacter actinomycetemcomitans. The pristine subgingival niches were quickly colonized by key pathogens. Their final concentration remained low, while the detection frequencies remained very high over time.ConclusionComplete edentulation results in a significant reduction of bacteria related to periodontitis and peri-implantitis, with the exception of A. actinomycetemcomitans, which might indicate that key pathogens can survive without pockets.