Accuracy of ELISA Detection Methods for Gluten and Reference Materials: A Realistic Assessment
Accuracy of ELISA Detection Methods for Gluten and Reference Materials: A Realistic Assessment
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DOI：
10.1021/jf3046736
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发表时间：
2013-06-19
影响因子：
6.1
通讯作者：
Popping, Bert
中科院分区：
文献类型：
--
作者：
Diaz-Amigo, Carmen;Popping, Bert
通过 ELISA 测定谷蛋白并随后将所得浓度转换为食品中的麸质含量似乎是一个相对简单和直接的过程，许多实验室在这方面拥有多年的经验。在该过程结束时，获得以 mg/kg 或 ppm 表示的面筋值。该值通常是决定产品是否可以贴上无麸质标签的基础。根据目前可用的科学信息，常用商业 ELISA 试剂盒获得的值的准确性值得怀疑。尽管最近进行了几项多实验室研究，试图强调和确保结果的准确性，但数据表明，得到证实的是这些测定的精确度，而不是准确性，因为计算麸质含量的一些基本假设缺乏科学数据支持以及用于比较的适当参考材料。本文讨论了面筋测定和定量方面的抗体特异性、提取程序、参考材料及其可互换性问题。
The determination of prolamins by ELISA and subsequent conversion of the resulting concentration to gluten content in food appears to be a comparatively simple and straightforward process with which many laboratories have years-long experience. At the end of the process, a value of gluten, expressed in mg/kg or ppm, is obtained. This value often is the basis for the decision if a product can be labeled gluten-free or not. On the basis of currently available scientific information, the accuracy of the obtained values with commonly used commercial ELISA kits has to be questioned. Although recently several multilaboratory studies have been conducted in an attempt to emphasize and ensure the accuracy of the results, data suggest that it was the precision of these assays, not the accuracy, that was confirmed because some of the underlying assumptions for calculating the gluten content lack scientific data support as well as appropriate reference materials for comparison. This paper discusses the issues of gluten determination and quantification with respect to antibody specificity, extraction procedures, reference materials, and their commutability.