High-resolution light microscopic characterization of mouse spermatogonia
High-resolution light microscopic characterization of mouse spermatogonia
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DOI：
10.1095/biolreprod65.4.1170
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发表时间：
2001-10-01
影响因子：
3.6
通讯作者：
Russell, LD
中科院分区：
文献类型：
--
作者：
Chiarini-Garcia, H;Russell, LD
使用塑料包埋组织的高分辨率光学显微镜并识别生精周期阶段的细胞，确定了 C57BL/6J 品系小鼠的精原细胞特征。每种精原细胞类型的预期频率是识别和分类各种细胞类型的主要因素。尽管描述了许多特征，但仍注意到精原细胞类型的几个主要差异。包括 A(s)、A(pr) 和 A(al) 精原细胞的组可以主要根据核膜内衬异染色质不存在的情况下异染色质的斑点来区分。 A(1) 细胞在整个细胞核中显示出细颗粒状染色质，并且沿核膜几乎没有异染色质薄片。 A(2) 到 A(4) 精原细胞含有逐渐增多的细胞核边缘异染色质。中间型精原细胞显示片状或浅异染色质，完全包围细胞核。 B型精原细胞沿核膜周期性地呈现圆形异染色质。使用灰度直方图可以对核特征进行客观量化，并显示灰度从四种细胞类型到 A(1) 细胞的逻辑转变为更窄和更暗的轮廓。区分精原细胞类型的能力是研究正常和异常精子发生中最早的生殖细胞类型的行为和动力学的先决条件。
Characteristics of spermatogonia were determined in the C57BL/6J strain mouse using high-resolution light microscopy of plastic-embedded tissues and identifying cells during stages of the spermatogenic cycle. The frequency of expecting each spermatogonial cell type was a major factor in identifying and categorizing various cell types. Although numerous characteristics were described, several major differences were noted in spermatogonial cell types. The group comprising A(s), A(pr), and A(al) spermatogonia could be differentiated based primarily on mottling of heterochromatin throughout the nucleus in the absence of heterochromatin lining the nuclear envelope. The A(1) cells displayed finely granular chromatin throughout the nucleus and virtually no flakes of heterochromatin along the nuclear membrane. The A(2) through A(4) spermatogonia contained progressively more heterochromatin rimming the nucleus. intermediate-type spermatogonia displayed flaky or shallow heterochromatin that completely rimmed the nucleus. Type B spermatogonia showed rounded heterochromatin periodically along the nuclear envelope. Use of gray-scale histograms allowed objective quantification of nuclear characteristics and showed a logical shift in the gray scale to a narrower and darker profile, from four cell types leading to A(1) cells. The ability to differentiate spermatogonial types is a prerequisite to studying the behavior and kinetics of the earliest of the germ cell types in both normal and abnormal spermatogenesis.