Pathogens of Bovine Respiratory Disease in North American Feedlots Conferring Multidrug Resistance via Integrative Conjugative Elements
Pathogens of Bovine Respiratory Disease in North American Feedlots Conferring Multidrug Resistance via Integrative Conjugative Elements
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DOI：
10.1128/jcm.02485-13
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发表时间：
2014-02-01
影响因子：
9.4
通讯作者：
McAllister, Tim A.
中科院分区：
文献类型：
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作者：
Klima, Cassidy L.;Zaheer, Rahat;McAllister, Tim A.
在这项研究中，我们确定了牛呼吸道疾病（BRD）相关的病毒和细菌病原体在牛中的患病率，并在收集的细菌中表征了遗传特征、抗菌药物的敏感性和抗菌药物耐药性决定簇的性质。使用PCR对来自阿尔伯塔、加拿大（n = 42）、德克萨斯州（n = 6）和内布拉斯加州（n = 20）的68例BRD死亡病例的鼻咽拭子和肺组织样本进行牛病毒性腹泻病毒（BVDV）、牛呼吸道合胞病毒、牛疱疹病毒1型、3型副流感病毒、牛支原体、溶血性曼氏杆菌、多杀性巴氏杆菌和睡眠嗜组织菌筛查。除牛疱疹病毒1型外，所有病原体均被检出。M.溶血性（91%）和BVDV（69%）是最流行的，在63%的牛中同时发生。分离的M.溶血性巴氏杆菌（n = 55）、多杀性巴氏杆菌（n = 8）和H.同时从肺中收集睡眠（n = 10）。在M.溶血性链球菌分离株，从内布拉斯加饲养场获得克隆亚群（n = 8）。所有三种细菌病原体都表现出很高的抗菌素耐药性，其中45%对三种或更多种抗菌素表现出耐药性。M.溶血性巴氏杆菌（n = 18）、多杀性巴氏杆菌（n = 3）和H.来自德克萨斯州和内布拉斯加州的somni（n = 3）具有整合接合元件（ICE），其赋予多达7种不同的抗菌剂类别的抗性。ICE从多杀性巴氏杆菌通过接合转移到大肠杆菌，从M. haemolytica和H.多杀性巴氏杆菌。ICE介导的细菌性BRD病原体的多药耐药谱可能是目前用于控制BRD的许多治疗性抗菌策略的主要损害。
In this study, we determined the prevalence of bovine respiratory disease (BRD)-associated viral and bacterial pathogens in cattle and characterized the genetic profiles, antimicrobial susceptibilities, and nature of antimicrobial resistance determinants in collected bacteria. Nasopharyngeal swab and lung tissue samples from 68 BRD mortalities in Alberta, Canada (n = 42), Texas (n = 6), and Nebraska (n = 20) were screened using PCR for bovine viral diarrhea virus (BVDV), bovine respiratory syncytial virus, bovine herpesvirus 1, parainfluenza type 3 virus, Mycoplasma bovis, Mannheimia haemolytica, Pasteurella multocida, and Histophilus somni. Excepting bovine herpesvirus 1, all agents were detected. M. haemolytica (91%) and BVDV (69%) were the most prevalent, with cooccurrence in 63% of the cattle. Isolates of M. haemolytica (n = 55), P. multocida (n = 8), and H. somni (n = 10) from lungs were also collected. Among M. haemolytica isolates, a clonal subpopulation (n = 8) was obtained from a Nebraskan feedlot. All three bacterial pathogens exhibited a high rate of antimicrobial resistance, with 45% exhibiting resistance to three or more antimicrobials. M. haemolytica (n = 18), P. multocida (n = 3), and H. somni (n = 3) from Texas and Nebraska possessed integrative conjugative elements (ICE) that conferred resistance for up to seven different antimicrobial classes. ICE were shown to be transferred via conjugation from P. multocida to Escherichia coli and from M. haemolytica and H. somni to P. multocida. ICE-mediated multidrug-resistant profiles of bacterial BRD pathogens could be a major detriment to many of the therapeutic antimicrobial strategies currently used to control BRD.