Effect of Qufengtongluo Decoction on PI3K/Akt Signaling Pathway in the Kidney of Type 2 Diabetes Mellitus Rat (GK Rat) with Diabetic Nephropathy.
Effect of Qufengtongluo Decoction on PI3K/Akt Signaling Pathway in the Kidney of Type 2 Diabetes Mellitus Rat (GK Rat) with Diabetic Nephropathy.
复制标题
祛风通络汤对2型糖尿病肾病大鼠(GK大鼠)肾脏PI3K/Akt信号通路的影响
DOI：
10.1155/2018/8421979
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Zhang YT
中科院分区：
文献类型：
--
作者：
Huang WJ;Fu Q;Xiao YH;Gong Q;Wu WJ;Shen ZL;Zhang H;Jia X;Huang XM;Zhang YX;Zhao JX;Wang SD;Jia M;Zhang YT
祛风通络汤是治疗糖尿病肾病的有效方剂。然而，其潜在的分子机制仍不清楚。在本研究中，我们试图探讨清热通络汤是否通过抑制PI3K/Akt信号通路发挥作用。将24只GK大鼠随机分为3组：空白组、假手术组、清热通络冲剂组。模型建立后，QFTL组大鼠灌胃给予QFTL汤，其余组给予纯净水灌胃。在为期8周的干预中，每2~3周检测24 小时尿蛋白。干预后取肾脏进行病理涂片、实时定量聚合酶链式反应和免疫印迹检测p-PI3K、p-Akt、PTEN、转化生长因子-β、PI3K、Akt、PTEN和转化生长因子-β的表达水平。清肾通络冲剂组大鼠肾纤维化程度轻于空白组，24 小时尿蛋白定量较空白组明显减少。与空白组比较，清热通络组p-PI3K、p-Akt、PI3K、Akt基因表达水平降低，PTEN、PTEN基因表达水平升高。此外，还可下调转化生长因子-β的表达。提示清肺利湿汤可能通过激活PTEN和抑制转化生长因子-β而抑制PI3K/Akt信号转导通路。
Qufengtongluo (QFTL) decoction is an effective treatment for diabetic nephropathy (DN). However, the underlying molecular mechanism is still unclear. In this study, we try to investigate whether QFTL decoction acts via inhibiting PI3K/Akt signaling pathway. Twenty-four GK rats were randomly divided into 3 groups: blank group, sham-operated group, and QFTL group. After model establishment, rats in QFTL group were given QFTL decoction by gavage, while the rest were given pure water. During the 8-week intervention, 24 hr urinal protein was measured every 2-3 weeks. After intervention, kidneys were removed for pathological smear, quantitative real-time PCR, and western blotting to detect expression levels of p-PI3K, p-Akt, PTEN, TGF-β, PI3K mRNA, Akt mRNA, PTEN mRNA, and TGF-β mRNA. QFTL group showed a slighter degree of renal fibrosis in Masson and PASM staining and a greater reduction of 24 hr urinal protein than blank group. Compared to blank group, expression levels of p-PI3K, p-Akt, PI3K mRNA, and Akt mRNA were lower in QFTL group, while expression levels of PTEN and PTEN mRNA were higher. Besides, TGF-β was downregulated by QFTL decoction. In conclusion, this study suggests that QFTL decoction might inhibit PI3K/Akt signaling pathway via activating PTEN and inhibiting TGF-β.
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影响因子：
4.6
作者：
Waasdorp M;Duitman J;Florquin S;Spek CA
通讯作者：
Spek CA
影响因子：
4.4
作者：
Patel, RK;Mohan, C
通讯作者：
Mohan, C
影响因子：
--
作者：
Ribback S;Cigliano A;Kroeger N;Pilo MG;Terracciano L;Burchardt M;Bannasch P;Calvisi DF;Dombrowski F
通讯作者：
Dombrowski F
影响因子：
3.7
作者：
Hao, Jun;Zhu, Lin;Duan, Huijun
通讯作者：
Duan, Huijun
影响因子：
3.4
作者：
Tang Wan-Xin;Cui Tian-Lei;Fu Ping
通讯作者：
Fu Ping