Aluminum tolerance in a spermidine synthase-overexpressing transgenic European pear is correlated with the enhanced level of spermidine via alleviating oxidative status
Aluminum tolerance in a spermidine synthase-overexpressing transgenic European pear is correlated with the enhanced level of spermidine via alleviating oxidative status
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DOI：
10.1016/j.envexpbot.2009.03.014
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发表时间：
2009-09
影响因子：
5.7
通讯作者：
X. Wen;Y. Ban;H. Inoue;N. Matsuda;T. Moriguchi
中科院分区：
文献类型：
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作者：
X. Wen;Y. Ban;H. Inoue;N. Matsuda;T. Moriguchi
铝（Al）胁迫是作物产量低下的主要原因，特别是在酸性土壤占主导地位的国家。为了验证多胺是否可以赋予铝耐性，以转基因欧洲梨（PyruscommunisL。对过表达苹果亚精胺合酶（MdSPDS 1）的品系#32和野生型（WT）进行30μM AlCl 3的长期胁迫。从株高、鲜重的净生长量和植株形态变化来看，32号品系的表现明显优于WT。尽管品系#32中的SPDS表达水平和亚精胺（Spd）滴度高于WT中的那些，这肯定是由于转基因（MdSPDS 1）表达，但在两个品系中的长期Al胁迫试验中均未观察到SPDS表达的进一步诱导。而胁迫后两品系的Spd滴度均显著增加。超氧化物歧化酶（SOD）或谷胱甘肽还原酶（GR）的活性以及脯氨酸或丙二醛（MDA）的积累在这种胁迫下改变，使转基因品系#32比WT更有利于存活。这些抗氧化参数与Spd效价密切相关。胁迫后32号品系的Ca和SOD的一些辅助因子金属含量明显高于WT。这些证据表明，Spd主要通过改善氧化状态以及影响矿物元素平衡来提高转基因株系32的铝胁迫耐受性。
Aluminum (Al) stress is a major cause of poor crop yields, particularly in those countries where acid soil predominate. To verify whether polyamine can confer Al tolerance, in vitro shoots of a transgenic European pear (Pyrus communis L. ‘Ballad’) line #32 overexpressing apple spermidine synthase (MdSPDS1) and the wild type (WT) were subjected to long-term stress for 30μM AlCl3. Based on net increment of shoot height (SHI) or fresh weight (FWI) and morphological changes upon the stress, the performance of line #32 was much better than that of WT. Although SPDS expression levels and spermidine (Spd) titers in line #32 were higher than those in WT, firmly due to the transgene (MdSPDS1) expression, no further induction of SPDS expression was observed from the long-term Al stress trial in both lines. While, Spd titers were considerably increased in both lines after the stress. The activities of superoxide dismutase (SOD) or glutathione reductase (GR) and the accumulation of proline or malondialdehyde (MDA) altered upon this stress toward a more favorable status for survival in the transgenic line #32 than in WT. These antioxidant parameters were closely related to Spd titer. Concentrations of calcium (Ca) and some co-factor metals of SOD in line #32 were diversely higher than that in WT after the stress. These evidences indicate that Spd is implicated in elevating of Al stress tolerance of the transgenic line #32 chiefly via ameliorating oxidative status as well as by affecting mineral element balance.