Presence of growth hormone-releasing factor-like immunoreactivity in rat duodenum.
Presence of growth hormone-releasing factor-like immunoreactivity in rat duodenum.
复制标题
大鼠十二指肠中存在生长激素释放因子样免疫反应性。
DOI：
10.1210/endo-117-4-1710
复制
发表时间：
1985
期刊：
影响因子：
4.8
通讯作者：
W. Vale
中科院分区：
文献类型：
--
作者：
T. Bruhn;R. T. Mason;W. Vale
用高特异性的大鼠生长激素释放因子（rGRF）放射免疫分析法（RIA）检测了部分纯化的大鼠下丘脑和十二指肠提取物中的GRF样免疫反应性（GRF-LI）。大鼠下丘脑中rGRF-LI的浓度为32.5 fmol/mg干重（455 fmol或3.18 ng/区域），而大鼠十二指肠中含有2.15 fmol/mg干重（111 fmol或0.78 ng/区域）。部分纯化的下丘脑和十二指肠提取物表现出位移的示踪剂平行rGRF。其他脑区（脑干、皮质）或胃肠道组织（前胃、胃窦、回肠、结肠、胰腺）GRF-LI水平均接近RIA检测水平。在凝胶过滤和反相HPLC系统中，下丘脑和十二指肠提取物中的rGRF-LI表现得像合成的rGRF。这两种组织提取物中含有的rGRF-LI与合成rGRF的共洗脱表明这些分子即使不相同，也是相似的。
Growth hormone-releasing factor-like immunoreactivity (GRF-LI) was detected in partially purified extracts of rat hypothalamus and duodenum with a highly specific RIA for rat GRF (rGRF). The concentration of rGRF-LI in the rat hypothalamus was 32.5 fmol/mg dry weight (455 fmol or 3.18 ng/region), while the rat duodenum contained 2.15 fmol/mg dry weight (111 fmol or 0.78 ng/region). Partially purified hypothalamic and duodenal extracts exhibited displacement of the tracer parallel to rGRF. GRF-LI levels in all other brain regions (brainstem, cortex) or gastrointestinal tract tissues (forestomach, antrum, ileum, colon, pancreas) measured were close to the detection level of the RIA. rGRF-LI in hypothalamic and duodenal extracts behaved like synthetic rGRF in both gel filtration and reverse-phase HPLC systems. Coelution of rGRF-LI contained in both these tissue extracts with synthetic rGRF suggests that these molecules are similar, if not identical.