MYB30 and ETHYLENE INSENSITIVE3 Antagonistically Modulate Root Hair Growth in Arabidopsis
MYB30 and ETHYLENE INSENSITIVE3 Antagonistically Modulate Root Hair Growth in Arabidopsis
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MYB30 和 ETHYLENE INSENSITIVE3 拮抗调节拟南芥根毛生长
DOI：
10.1111/tpj.15180
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发表时间：
2021
期刊：
影响因子：
7.2
通讯作者：
Huapeng Zhou
中科院分区：
文献类型：
--
作者：
Fei Xiao;Qianyuan Gong;Shuangshuang Zhao;Honghui Lin;Huapeng Zhou
根毛（RH）是植物获取养分和植物与环境通讯的必需物质。在这里，我们报告的转录因子MYB 30和乙烯不敏感3（EIN 3）调节RH生长/伸长在拟南芥中以拮抗的方式。MYB 30功能丧失突变体显示RH长度增加，而MYB 30过表达植物中的RH伸长被高度抑制。MYB 30与乙烯信号传导中的主转录因子EIN 3物理相互作用。MYB 30直接结合到根毛缺陷六样蛋白4（RSL 4）的启动子区并抑制其转录，RSL 4功能缺失抑制了myb 30突变体中RH的生长。乙烯促进MYB 30-EIN 3复合物的形成，并通过EIN 3的作用降低MYB 30与RSL 4启动子之间的缔合。MYB 30和EIN 3以全基因组方式拮抗调节RSL 4和核心RH基因子集的表达。综上所述，我们的工作揭示了一种新的转录网络，调节植物中的RH生长。
Root hair (RH) is essential for plant nutrient acquisition and the plant–environment communication. Here we report that transcription factors MYB30 and ETHYLENE INSENSITIVE3 (EIN3) modulate RH growth/elongation in Arabidopsis in an antagonistic way. The MYB30 loss‐of‐function mutant displays enhanced RH length, whereas the RH elongation in MYB30‐overexpressing plants is highly repressed. MYB30 physically interacts with EIN3, a master transcription factor in ethylene signaling. MYB30 directly binds the promoter region ofROOT HAIR DEFECTIVE SIX‐LIKE4(RSL4) and represses its transcription.RSL4loss‐of‐function suppresses the enhanced RH growth inmyb30mutant plants. Ethylene enhances MYB30‐EIN3 complex formation, and reduces the association between MYB30 andRSL4promotor via the action of EIN3. MYB30 and EIN3 antagonistically regulate the expression ofRSL4and a subset of core RH genes in a genome‐wide way. Taken together, our work revealed a novel transcriptional network that modulates RH growth in plants.