Constitutive expression of ICAM-1 in rat microvascular systems analyzed by laser confocal microscopy
Constitutive expression of ICAM-1 in rat microvascular systems analyzed by laser confocal microscopy
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DOI：
10.1152/ajpheart.1997.273.1.h138
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发表时间：
1997-07-01
影响因子：
4.8
通讯作者：
Takashi, T
中科院分区：
文献类型：
--
作者：
Iigo, Y;Suematsu, M;Takashi, T
本研究旨在证实细胞间黏附分子-1(ICAM-1)在大鼠肠系膜和肝脏的小动脉、毛细血管和小静脉中的组成性表达，并观察培养的内皮细胞和白细胞之间的相互作用。激光共聚焦荧光显微镜观察ICAM-1在体内微血管中的表达。用异硫氰酸荧光素标记大鼠细胞间黏附分子-1(1A29)的单抗，测定荧光与免疫球蛋白的结合率进行数据校正。血管内注射异硫氰酸荧光素标记的1A29在肠系膜微血管中分布不均：毛细血管后微静脉是ICAM-1的主要表达部位，与其内皮结合的1A29的密度至少是同一肠系膜内真毛细血管和小动脉的10倍以上。肝细胞间黏附分子-1在肝窦内大量表达，其表达程度与中央小静脉相似。这些结果表明，在急性炎症中，毛细血管后微静脉是一种主动的通道，可以帮助粘连暴露于促炎刺激的循环白细胞。
The present study aimed to demonstrate constitutive expression of the intercellular adhesion molecule (ICAM)-1 among arterioles, capillaries, and venules in the mesentery and liver and to examine the interaction between cultured endothelial cells and leukocytes in rats. ICAM-1 expression in the microvessels in vivo was visually demonstrated by laser confocal fluorescence microscopy. A monoclonal antibody against rat ICAM-1 (1A29) was labeled with fluorescein isothiocyanate, and the binding ratio between the fluorescence and immunoglobulin was determined for data calibration. Intravascularly administered fluorescein isothiocyanate-labeled 1A29 was distributed heterogeneously among the hierarchy of microvessels in the mesentery: postcapillary venules were the major portion expressing ICAM-1 constitutively, and the density of 1A29 bound to their endothelium was at least 10 times higher than that in true capillaries and arterioles in the same mesentery. On the other hand, the liver expressed ICAM-1 abundantly in sinusoids to the extent similar to that in central venules. These results suggest that postcapillary venules serve as an active gateway with the readiness to help adhere circulating leukocytes exposed to proinflammatory stimuli in acute inflammation.