General pattern of meiotic recombination in male dogs estimated by MLH1 and RAD51 immunolocalization
General pattern of meiotic recombination in male dogs estimated by MLH1 and RAD51 immunolocalization
复制标题
DOI：
10.1007/s10577-008-1221-y
复制
发表时间：
2008-08-01
影响因子：
2.6
通讯作者：
Borodin, P. M.
中科院分区：
文献类型：
--
作者：
Basheva, E. A.;Bidau, C. J.;Borodin, P. M.
本研究的目的是估计一般模式的减数分裂重组的家犬（犬）使用MLH 1，成熟的重组结节的错配修复蛋白的免疫定位。我们从三只雄性狗的124个精母细胞中制备了联会复合体（SC），并沿着沿着4712个常染色体绘制了4959个MLH 1灶。所有常染色体的MLH 1病灶平均数量为每个细胞40.0个病灶。雄性犬常染色体基因组图谱的总重组长度估计为2000 cM。MLH 1病灶分布沿着常染色体二价体的全球模式是相当相似的，在哺乳动物中发现的研究：高频率附近的端粒和低频率附近的着丝粒。X-Y配对区域中的专性MLH 1焦点通常位于非常靠近Xp-Yq端粒的位置。常染色体二价体MLH 1病灶之间的距离与交叉干扰一致。来自MLH 1焦点间距离和RAD 51/MLH 1焦点比分布的干扰估计值的比较表明，物种之间的干扰强度存在很大差异。
The aim of this study was to estimate a general pattern of meiotic recombination in the domestic dog (Canis familiaris) using immunolocalization of MLH1, a mismatch repair protein of mature recombination nodules. We prepared synaptonemal complex (SC) spreads from 124 spermatocytes of three male dogs and mapped 4959 MLH1 foci along 4712 autosomes. The mean number of MLH1 foci for all autosomes was 40.0 foci per cell. Total recombination length of the male dog autosomal genome map was estimated as 2000 cM. A global pattern of MLH1 foci distribution along the autosomal bivalents was rather similar to that found in the mammals studied: a high frequency near the telomeres and a low frequency near the centromeres. An obligate MLH1 focus in the X-Y pairing region was usually located very close to Xp-Yq telomeres. The distances between MLH1 foci at autosomal bivalents were consistent with crossover interference. A comparison of the interference estimates coming from the distribution of MLH1 interfocus distances and RAD51/MLH1 focus ratio indicated a substantial variation between species in the strength of interference.