Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage
Use of autologous adipose-derived mesenchymal stem cells for creation of laryngeal cartilage
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DOI：
10.1002/lary.26980
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发表时间：
2018-04-01
期刊：
影响因子：
2.6
通讯作者：
Halum, Stacey
中科院分区：
文献类型：
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作者：
Zhang, Hongji;Voytik-Harbin, Sherry;Halum, Stacey
目的/假设脂肪间充质干细胞（adipose-derived mesenchymal stem cells，ASCs）是组织工程的一个令人兴奋的潜在细胞来源，因为细胞可以从简单的自体脂肪切除中获得。本研究介绍了一种从ASC和定制的胶原低聚物溶液中进行组织工程软骨的新方法，并证明了所得软骨可用于动物模型中的喉软骨重建。研究设计基础科学实验设计方法从F344大鼠中分离ASC，接种在定制的胶原基质中，并在软骨分化培养基中培养1，2，4周，直到在体外显示出软骨样特征。在F344大鼠模型中制造大的喉软骨缺损，用工程化软骨替代软骨缺损，并且大鼠随访1至3个月。染色检查细胞形态和软骨特异性features.ResultsIn体外组织学染色显示圆形软骨细胞出现的细胞均匀地居住在整个定制的胶原蛋白支架，与软骨特异性标志物的阳性染色。软骨被用来成功地修复大的软骨缺损的大鼠model.ConclusionsThis研究是第一个研究证明，在动物模型中，ASCs培养在一个独特的形式的胶原低聚物可以创建功能的软骨样移植物，可以成功地用于部分喉软骨置换。
Objectives/HypothesisAdipose-derived mesenchymal stem cells (ASCs) are an exciting potential cell source for tissue engineering because cells can be derived from the simple excision of autologous fat. This study introduces a novel approach for tissue-engineering cartilage from ASCs and a customized collagen oligomer solution, and demonstrates that the resultant cartilage can be used for laryngeal cartilage reconstruction in an animal model.Study DesignBasic science experimental design.MethodsASCs were isolated from F344 rats, seeded in a customized collagen matrix, and cultured in chondrogenic differentiation medium for 1, 2, and 4 weeks until demonstrating cartilage-like characteristics in vitro. Large laryngeal cartilage defects were created in the F344 rat model, with the engineered cartilage used to replace the cartilage defects, and the rats followed for 1 to 3 months. Staining examined cellular morphology and cartilage-specific features.ResultsIn vitro histological staining revealed rounded chondrocyte-appearing cells evenly residing throughout the customized collagen scaffold, with positive staining for cartilage-specific markers. The cartilage was used to successfully repair large cartilaginous defects in the rat model, with excellent functional results.ConclusionsThis study is the first study to demonstrate, in an animal model, that ASCs cultured in a unique form of collagen oligomer can create functional cartilage-like grafts that can be successfully used for partial laryngeal cartilage replacement.