Modulation of cell adhesion and viability of cultured murine bone marrow cells by arsenobetaine, a major organic arsenic compound in marine animals
Modulation of cell adhesion and viability of cultured murine bone marrow cells by arsenobetaine, a major organic arsenic compound in marine animals
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砷甜菜碱（海洋动物中的一种主要有机砷化合物）对培养的小鼠骨髓细胞的细胞粘附和活力的调节
DOI：
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发表时间：
2001
影响因子：
7.3
通讯作者：
K. Fujiwara
中科院分区：
文献类型：
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作者：
T. Sakurai;K. Fujiwara
本研究利用小鼠骨髓（BM）细胞研究了三甲基（羧甲基）砷两性离子（即砷甜菜碱（AsBe））对海洋动物的生物效应，并与无机砷（亚砷酸钠）的体外生物学效应进行了比较。亚砷酸钠对BM细胞有较强的细胞毒性，其IC50为6 μM。相比之下，AsBe在72小时的孵育过程中以剂量依赖性的方式显著增强了BM细胞的活力；当AsBe浓度为μM时，细胞活力比单独用该培养基培养的对照细胞提高约2倍。在形态学研究中，在72小时的BM培养过程中，AsBe增加了大的成熟贴壁细胞的数量，尤其是粒细胞。当BM细胞与AsBe和低剂量（1 μ ml−1）重组小鼠粒细胞/巨噬细胞集落刺激因子（rMu GM - CSF）一起培养时，观察到BM细胞中粒细胞和巨噬细胞的数量显著增加。然而，通过凝胶培养基的集落形成实验确定，AsBe完全不会引起BM细胞的增殖。这些发现表明AsBe在哺乳动物细胞中具有独特而强大的生物效应，AsBe是许多国家每天作为海产品摄入的各种海洋动物中的一种主要有机砷化合物。
In this study, we investigated the biological effects of trimethyl (carboxymethyl) arsonium zwitterion, namely arsenobetaine (AsBe), which is a major organic arsenic compound in marine animals using murine bone marrow (BM) cells and compared them with those of an inorganic arsenical, sodium arsenite, in vitro. Sodium arsenite showed strong cytotoxicity in BM cells, and its IC50 was 6 μM. In contrast, AsBe significantly enhanced the viability of BM cells in a dose‐dependent manner during a 72‐h incubation; about a twofold increase in the viability of cells compared with that of control cells cultured with the medium alone was observed with a μM level of AsBe. In morphological investigations, AsBe enhanced the numbers of large mature adherent cells, especially granulocytes, during a 72‐h BM culture. When BM cells were cultured together with AsBe and a low dose (1 u ml−1) of recombinant murine granulocyte/macrophage colony‐stimulating factor (rMu GM‐CSF), significant additive‐like increasing effects were observed on the numbers of both granulocytes and macrophages originated from BM cells. However, AsBe did not cause proliferation of BM cells at all as determined by colony‐forming assay using a gelatious medium. These findings demonstrate the unique and potent biological effects in mammalian cells of AsBe, a major organic arsenic compound in various marine animals which are ingested daily as seafood in many countries.
砷化合物的酶促甲基化：兔肝亚砷酸甲基转移酶和单甲基胂酸甲基转移酶的测定、部分纯化和性质。
影响因子：
4.1
作者：
Zakharyan,R;Wu,Y;Bogdan,GM;Aposhian,HV
通讯作者：
Aposhian,HV