Anti-cancer effect of hyperthermia on breast cancer by magnetite nanoparticle-loaded anti-HER2 immunoliposomes
Anti-cancer effect of hyperthermia on breast cancer by magnetite nanoparticle-loaded anti-HER2 immunoliposomes
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DOI：
10.1007/s10549-008-9948-x
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发表时间：
2009-02-01
影响因子：
3.8
通讯作者：
Imai, Tsuneo
中科院分区：
文献类型：
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作者：
Kikumori, Toyone;Kobayashi, Takeshi;Imai, Tsuneo
背景我们已经构建了含有磁铁矿纳米颗粒（HML）的抗HER2免疫脂质体，该纳米颗粒在交变磁场（AMF）中产生热量。使用细胞培养模型已经实现了HML的有效靶向和杀细胞能力。本研究的目的是探讨这种模式的可行性乳腺癌治疗荷瘤小鼠模型。材料与方法采用BT474（HER 2高表达）或SKOV 3（HER 2低表达）细胞的裸小鼠皮下癌结节作为模型。将HML注射到这些癌结节中，然后以24小时的间隔暴露于AMF两次，持续30分钟。检查磁铁矿的积累和肿瘤生长速率。还检查了热效应的组织学结果。结果HML仅在BT474肿瘤中聚集。肿瘤温度上升到45摄氏度，而体温保持在38摄氏度左右。热疗组肿瘤消退，热疗后持续10周。结论HML热疗是治疗HER2高表达乳腺癌的一种有效而特异的方法。这种疗法可能提供一种替代方法来治疗其他方式难治的复发性癌症。
Background We have constructed anti-HER2 immunoliposomes containing magnetite nanoparticles (HML) that generate heat in an alternating magnetic field (AMF). The effective targeting and cytocidal abilities of HML have been achieved using cell culture models. This study aimed to investigate feasibility of this modality for breast cancer treatment using tumor-bearing mouse models. Material and methods The subcutaneous cancer nodules of BT474 (high HER2 expression) or SKOV3 (low HER2 expression) cells in nude mice were employed as models. HMLs were injected into these cancer nodules and were then exposed to an AMF for 30 min twice at 24 h intervals. Accumulation of magnetite and tumor growth rates were examined. Histological findings of the thermal effect were also examined. Results HMLs accumulated in only BT474 tumors. The tumor temperature increased to 45A degrees C whereas the body temperature remained at around 38A degrees C. Tumor regression was observed in the hyperthermic group and was sustained for 10 weeks after hyperthermia. Conclusion These results suggest that hyperthermia using HML is an effective and specific therapy for breast cancer overexpressing HER2. This therapy may provide an alternative way to treat recurrent cancer refractory to other modalities.