Polymer-fiber-coupled field-effect sensors for label-free deep brain recordings
Polymer-fiber-coupled field-effect sensors for label-free deep brain recordings
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DOI：
10.1371/journal.pone.0228076
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发表时间：
2020-01-24
期刊：
影响因子：
3.7
通讯作者：
Yoshinobu, Tatsuo
中科院分区：
文献类型：
--
作者：
Guo, Yuanyuan;Werner, Carl F.;Yoshinobu, Tatsuo
电记录允许直接读出神经活动，但提供的能力有限，无法将其与网络拓扑相关联。另一方面，光学成像揭示了神经电路的结构，但依赖于笨重的光学元件和荧光记录器，其信号被脑组织衰减。在这里，我们介绍了基于场效应的可植入设备来记录大脑活动，这种设备可以进一步扩展，具有无标记电生理标测的能力。这类设备依靠光可寻址电位传感器(LAPS)，与集成电极和光波导束的聚合物纤维相连。LAPS利用场效应将电生理活动转化为区域载流子重新分布，并以空间分辨的方式将神经活动读出为由调制光束诱导的光电流。空间分辨光电流记录是通过照射光纤束中的不同像素来实现的。这些设备被应用于记录小鼠海马区的局部场电位。与通过单一调制波束的栅格扫描相结合，这项技术可以实现对大脑深层区域神经活动的快速无标记成像。
Electrical recording permits direct readout of neural activity but offers limited ability to correlate it to the network topography. On the other hand, optical imaging reveals the architecture of neural circuits, but relies on bulky optics and fluorescent reporters whose signals are attenuated by the brain tissue. Here we introduce implantable devices to record brain activities based on the field effect, which can be further extended with capability of label-free electrophysiological mapping. Such devices reply on light-addressable potentiometric sensors (LAPS) coupled to polymer fibers with integrated electrodes and optical waveguide bundles. The LAPS utilizes the field effect to convert electrophysiological activity into regional carrier redistribution, and the neural activity is read out in a spatially resolved manner as a photocurrent induced by a modulated light beam. Spatially resolved photocurrent recordings were achieved by illuminating different pixels within the fiber bundles. These devices were applied to record local field potentials in the mouse hippocampus. In conjunction with the raster-scanning via the single modulated beam, this technology may enable fast label-free imaging of neural activity in deep brain regions.