Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
Determination of antiviral action of long non-coding RNA loc107051710 during infectious bursal disease virus infection due to enhancement of interferon production
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DOI：
10.1080/21505594.2019.1707957
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发表时间：
2020-01-01
期刊：
影响因子：
5.2
通讯作者：
Tang, Lijie
中科院分区：
文献类型：
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作者：
Huang, Xuewei;Xu, Yigang;Tang, Lijie
传染性法氏囊病病毒（IBDV）感染过程中lncrna的功能和特征尚未确定。本研究的目的是通过研究loc107051710与IRF8、I型IFN、STATs和ISGs之间的关系，确定loc107051710 lncRNA在IBDV感染期间的抗病毒作用。DF-1细胞作为未感染的对照组（n = 1）或感染IBDV的细胞（n = 3）不进行处理。采用RNA测序法分析mrna和lncRNAs的表达。采用RT-qPCR验证差异表达基因。然后通过两两比较，对感染组和对照组的230个表达差异显著的基因（182个mrna和48个lncRNA）进行鉴定。通过选择上述生物过程和信号通路中显著富集的lncrna和mrna，构建lncRNA-mRNA共表达网络，研究差异表达lncrna的功能。应用了基因本体技术和京都基因与基因组百科全书路径技术。结果表明，这些差异表达基因参与了宿主与IBDV的相互作用。发现Loc107051710具有潜在的抗病毒作用。应用RT-qPCR和western blot检测发现，loc107051710是诱导IRF8、I型IFN、STAT和ISG表达所必需的，其敲低可促进IBDV复制。荧光原位杂交发现，感染IBDV后，loc107051710从细胞核转移到细胞质中。总的来说，loc107051710通过调节IRF8促进ifn - α和ifn - β的产生，从而促进ISGs的抗病毒活性。
The functions and profiles of lncRNAs during infectious bursal disease virus (IBDV) infection have not been determined, yet. The objectives of this study were to determine the antiviral action of loc107051710 lncRNA during IBDV infection by investigating the relationship between loc107051710 and IRF8, Type I IFN, STATs, and ISGs. DF-1 cells were either left untreated as non-infected controls (n = 1) or infected with IBDV (n = 3). RNA sequencing was applied for analysis of mRNAs and lncRNAs expression. Differentially expressed genes were verified by RT-qPCR. Then identification, of 230 significantly different expressed genes (182 mRNAs and 48 lncRNA) by pairwise comparison of the infected and control groups, was carried out. The functions of differentially expressed lncRNAs were investigated by selection of lncRNAs and mRNAs significantly enriched in the aforementioned biological processes and signaling pathways for construction of lncRNA-mRNA co-expression networks. The techniques of gene ontology and Kyoto Encyclopedia of Genes and Genomes pathways were applied. It was suggested that these differentially expressed genes were involved in the interaction between the host and IBDV. Loc107051710 was found to have potential antiviral effects. RT-qPCR and western blot were applied and revealed that loc107051710 was required for induction of IRF8, type I IFN, STAT, and ISG expression, and its knockdown promoted IBDV replication. By fluorescence in situ hybridization, it was found that loc107051710 was translocated from the nucleus to the cytoplasm after infection with IBDV. Overall, loc107051710 promoted the production of IFN-alpha and IFN-beta by regulating IRF8, thereby promoting the antiviral activity of ISGs.