Experimental study on repairing of nude mice skin defects with composite skin consisting of xenogeneic dermis and epidermal stem cells and hair follicle dermal papilla cells
Experimental study on repairing of nude mice skin defects with composite skin consisting of xenogeneic dermis and epidermal stem cells and hair follicle dermal papilla cells
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DOI：
10.1016/j.burns.2007.04.003
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发表时间：
2008-05-01
期刊：
影响因子：
2.7
通讯作者：
Tian-Zenq, Li
中科院分区：
文献类型：
--
作者：
Shao-Hai, Qi;Po, Liu;Tian-Zenq, Li
目的：探讨毛囊真皮乳头细胞（DPCs）对复合皮肤生物学特性的影响。
方法：实验组以异种脱细胞真皮基质为支架，将DPCs接种于真皮支架的皮下侧，上皮干细胞接种于真皮乳头侧，构建复合皮肤。对照组支架中无DPC。将两种复合皮肤用于覆盖裸鼠背部皮肤缺损。移植4周后观察创面愈合情况，分析面积并计算收缩率。取移植区组织标本进行HE染色，观察复合皮肤状态。测量取材皮肤的应力 - 应变曲线，以计算样本的最大断裂力。收集数据并进行统计学分析。
结果：HE染色显示实验组上皮厚度增加（细胞层数超过10层），对照组仅6 - 7层。皮肤移植后第4周，实验组皮肤收缩率（3.94±0.013）%远低于对照组（29.07±0.018）%（P < 0.05）。生物力学测试表明，与对照组相比，实验组复合皮肤的应力 - 应变曲线更接近正常裸鼠皮肤。实验组复合皮肤的最大断裂力为（1.835±0.035）N（牛顿），对照组为（1.075±0.065）N（P < 0.01）。
结论：接种于真皮基质支架的真皮DPCs促进了复合皮肤中表皮的重建。因此，含DPCs的复合皮肤收缩较小，皮肤的生物学特性得到改善。（C）2007爱思唯尔有限公司和国际烧伤学会。版权所有。
Objective: To investigate the influence of hair follicle dermal papilla cells (DPCs) on biological features of composite skin.Methods: In the test group, xenogeneic acellular dermal matrix was employed as the frame, DPCs were seeded on the subcutaneous side, and epithelial stem cells onto the dermal papilla side of the dermal frame so as to construct a composite skin. in the control group, there was no DPC in the frame. The two kinds of composite skin were employed to cover skin defects on the back of the nude mice. Wound healing was observed 4 weeks after grafting and area was analyzed and contraction rate was calculated. The tissue samples in the grafted area were harvested for HE staining and the state of the composite skin was observed. The stress-strain curve of the sampled skin was measured, so as to calculate the maximal breaking power of the sample. The data were collected and statistically analyzed.Results: HE staining indicated that the epithelial depth was increased (more than 10 layers of cells) in test group, with only 6-7 layers in control group. The skin contraction rate in test group on the 4th week after skin grafting (3.94 +/- 0.013)% was much lower than that in control group (29.07 +/- 0.018)% (P < 0.05). it was indicated by biomechanical test that the stress-strain curve of the composite skin in the test group was closer to that of normal nude mice skin in comparison to that in control group. The maximal breaking force of the composite skin in test group was (1.835 +/- 0.035) N (Newton), while that in control group was (1.075 +/- 0.065) N (P < 0.01).Conclusion: Reconstruction of epidermis in composite skin was promoted by dermal DPCs seeded in the dermal matrix frame. As a result, there was less skin contraction in the composite skin with DPCs, so that the biological characteristics of the skin were improved. (C) 2007 Elsevier Ltd and ISBI. All rights reserved.