Pityriasis rosea is associated with systemic active infection with both human herpesvirus-7 and human herpesvirus-6
Pityriasis rosea is associated with systemic active infection with both human herpesvirus-7 and human herpesvirus-6
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DOI：
10.1046/j.1523-1747.2002.00200.x
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发表时间：
2002-10-01
影响因子：
6.5
通讯作者：
Blauvelt, A
中科院分区：
文献类型：
--
作者：
Watanabe, T;Kawamura, T;Blauvelt, A
玫瑰糠疹是一种常见的皮肤病，一直怀疑有病毒病因。我们采用巢式聚合酶链反应检测了14例玫瑰糠疹患者的病变皮肤、非病变皮肤、外周血单个核细胞、血清和唾液样本中的人疱疹病毒-7、人疱疹病毒-6和巨细胞病毒DNA。采用原位杂交技术和透射电镜技术分别研究了病变皮肤内病毒mRNA的表达和病毒粒子的可视化。通过巢式聚合酶链反应，人疱疹病毒-7 DNA存在于病变皮肤（93%）、非病变皮肤（86%）、唾液（100%）、外周血单核细胞（83%）和血清（100%）样本中，而人疱疹病毒-6 DNA存在于病变皮肤（86%）、非病变皮肤（79%）、唾液（80%）、外周血单核细胞（83%）和血清（88%）样本中。相比之下，在这些组织中未检测到巨细胞病毒DNA。来自12名健康志愿者和10名牛皮癣患者的对照样本在皮肤或血清中显示罕见的人类疱疹病毒-7或人类疱疹病毒-6 DNA阳性。通过原位杂交，在100%和75%的玫瑰糠疹皮损的血管周围和附件周围区分别鉴定出表达人疱疹病毒-7和人疱疹病毒-6 mRNA的浸润性单核细胞，而正常皮肤和牛皮癣皮肤对照组中只有13%的疱疹病毒mRNA阳性。透射电子显微镜未能显示玫瑰糠疹病变皮肤中的疱疹病毒病毒体。巢式聚合酶链反应和原位杂交技术可以在玫瑰糠疹患者的皮肤和其他组织中检测到人疱疹病毒-7和人疱疹病毒-6。这些结果表明玫瑰糠疹与人类疱疹病毒-7和人类疱疹病毒-6的全身活动性感染有关。
Pityriasis rosea is a common skin disease that has been suspected to have a viral etiology. We performed nested polymerase chain reaction to detect human herpesvirus-7, human herpesvirus-6, and cytomegalovirus DNA in lesional skin, nonlesional skin, peripheral blood mononuclear cells, serum, and saliva samples isolated from 14 pityriasis rosea patients. Viral mRNA expression and virion visualization within lesional skin were studied by in situ hybridization and transmission electron microscopy, respectively. By nested polymerase chain reaction, human herpesvirus-7 DNA was present in lesional skin (93%), nonlesional skin (86%), saliva (100%), peripheral blood mononuclear cells (83%), and serum (100%) samples, whereas human herpesvirus-6 DNA was detected in lesional skin (86%), nonlesional skin (79%), saliva (80%), peripheral blood mononuclear cells (83%), and serum (88%) samples. By contrast, cytomegalovirus DNA was not detected in these tissues. Control samples from 12 healthy volunteers and 10 psoriasis patients demonstrated rare positivity for either human herpesvirus-7 or human herpesvirus-6 DNA in skin or serum. By in situ hybridization, infiltrating mononuclear cells expressing human herpesvirus-7 and human herpesvirus-6 mRNA were identified in perivascular and periappendageal areas in 100% and 75% pityriasis rosea skin lesions, respectively, compared to herpesviral mRNA positivity in only 13% normal skin and psoriasis skin controls. Transmission electron microscopy failed to reveal herpesviral virions in pityriasis rosea lesional skin. Nested polymerase chain reaction and in situ hybridization enabled detection of human herpesvirus-7 and human herpesvirus-6 in skin and other tissues isolated from patients with pityriasis rosea. These results suggest that pityriasis rosea is associated with systemic active infection with both human herpesvirus-7 and human herpesvirus-6.