Expression of hepatocyte growth factor and c-met in ulcerative colitis
Expression of hepatocyte growth factor and c-met in ulcerative colitis
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DOI：
10.1007/pl00000212
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发表时间：
2000-07-01
影响因子：
6.7
通讯作者：
Matsuzawa, Y
中科院分区：
文献类型：
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作者：
Kitamura, S;Kondo, S;Matsuzawa, Y
目标与设计：本研究旨在确定肝细胞生长因子（HGF）-Met系统是否参与溃疡性结肠炎（UC）炎症粘膜的修复过程以及UC相关结直肠癌的发生。材料和方法。采用竞争性逆转录-聚合酶链反应定量检测健康对照组、UC患者和UC相关结直肠癌患者的结肠粘膜标本中HGF和c-met基因表达。免疫印迹法检测肝细胞生长因子蛋白表达。结果：UC患者炎症黏膜组织中HGF和c-met基因表达均较正常对照组明显增高。肝细胞生长因子的基因表达也增加了周围发炎粘膜的UC相关的癌症。在HGF基因表达增加的情况下，通过免疫印迹分析观察到UC炎症粘膜中HGF蛋白水平明显增加。c-met基因在UC相关的癌症中过表达，并且c-Met蛋白的免疫反应性在癌细胞内被免疫组化检测到。结论：我们表明，HGF和c-met表达在UC的炎症粘膜中增加，并且c-met在UC相关的结直肠癌中过表达。这些观察结果表明HGF-Met系统参与UC炎症粘膜的修复过程，并进一步支持了UC患者中HGF和c-met基因的不适当表达易患结直肠癌的观点。
Objective and Design: This study was designed to determine if the hepatocyte growth factor (HGF)-Met system is involved in the repair process of inflamed mucosa of ulcerative colitis (UC) and in the development of UC-associated colorectal cancer.Materials and Methods. HGF and c-met gene expressions were quantified in colonic mucosal specimens from healthy control subjects, patients with UC and patients with UC-associated colorectal cancer, using the competitive reverse transcription-polymerase chain reaction. Expression of HGF protein was determined by immunoblot analysis. Expression of c-Met protein was analyzed immunohistochemically.Results: HGF and c-met gene expressions were increased in inflamed mucosa of UC, compared with control subjects. Gene expression of HGF was also increased in the surrounding inflamed mucosa of UC-associated cancers. In cases in which the HGF gene expression was increased, an apparent increase in protein levels of HGF in inflamed mucosa of UC were observed by immunoblot analysis. The c-met gene was overexpressed in UC-associated cancers and a high level of immunoreactivity of the c-Met protein was immunohistochemically detected within the cancer cells.Conclusion: We showed that HGF and c-met expression is increased in the inflamed mucosa of UC and that c-met is overexpressed in UC-associated colorectal cancers. These observations suggest HGF-Met system is involved in the repair process of the inflamed mucosa of UC and provide further support for the view that the inappropriate expressions of both HGF and c-met genes predispose to the development of colorectal cancer in patients with UC.