Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex.
Chemically and photochemically initiated DNA cleavage by an insulin-mimetic bisperoxovanadium complex.
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通过类胰岛素双过氧钒复合物以化学和光化学方式引发 DNA 裂解。
DOI：
10.1007/bf01075916
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发表时间：
1995
影响因子：
4.3
通讯作者：
Dabrowiak,JC
中科院分区：
文献类型：
--
作者：
Hiort,C;Goodisman,J;Dabrowiak,JC
研究了类胰岛素化合物NH4[VO(O2)2-(1，10-邻菲咯啉)]，BPV(Phen)对DNA的化学和光化学切割作用。~(51)V核磁共振和吸收光谱表明，含BPV(Phen)的水溶液在低能紫外光照射下，使该化合物转化为简单的钒酸盐。在超螺旋pBR322 DNA存在的情况下，对该化合物进行光照射会导致切割该质粒，以产生有缺口的环状和线状DNA。琼脂糖凝胶数据的定量分析表明，BPV(Phen)是一种具有序列和/或碱基特异性的单链划痕剂。
Chemically and photochemically induced cleavage of DNA by the insulin-mimetic compound NH4[VO(O2)2-(1,10-phenanthroline)], bpV(phen), have been studied.51V NMR and absorption indicate that photoirradiation with low energy UV light of aqueous solutions containing bpV(phen) leads to the conversion of the compound to simple vanadates. Photoillumination of the compound in the presence of supercoiled pBR322 DNA results in cutting of the plasmid to produce nicked circular and linear DNA. Quantitative analysis of agarose gel data shows that bpV(phen) is a single strand nicking agent exhibiting sequence and/or base specificity.