Dual-Functionalized Apatite Nanocomposites with Enhanced Cytocompatibility and Osteogenesis for Periodontal Bone Regeneration
Dual-Functionalized Apatite Nanocomposites with Enhanced Cytocompatibility and Osteogenesis for Periodontal Bone Regeneration
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具有增强细胞相容性和成骨作用的双功能磷灰石纳米复合材料用于牙周骨再生
DOI：
10.1021/acsbiomaterials.9b01893
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发表时间：
2020
影响因子：
--
通讯作者：
Jinlin Song
中科院分区：
文献类型：
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作者：
MingLi Xiang;Mengyuan Zhu;Zun Yang;Ping He;Jingjing Wei;Xiang Gao;Jinlin Song
牙周组织工程仿生骨移植材料的研究是一个热点领域。在这项研究中，我们设计了一种双功能化的磷灰石纳米复合材料，它可以整合多种分子线索来操纵牙周膜干细胞（PDLSCs）的命运。简而言之，受贻贝的启发，使用聚多巴胺结构作为模板（命名为tHA）制造仿生纳米羟基磷灰石，然后通过儿茶酚化学的单一步骤用骨形成肽-1（BFP-1）和血管内皮生长因子模拟肽（QK）进行表面修饰。我们的研究表明，在磷灰石纳米颗粒表面的拴系肽的生物功能没有受到损害。由于BFP-1和QK肽的协同作用，双功能化磷灰石纳米复合材料与对照相比显示出改善的细胞相容性。此外，它可以促进PDLSC的增殖和成骨分化，表明tHA-BFP/QK纳米颗粒对细胞命运决定具有优异的生物活性。更重要的是，动物实验表明，双功能化磷灰石纳米复合材料可以显着促进牙周骨再生。我们的工作为仿生磷灰石纳米复合材料的设计提供了有益的启示，该材料在牙周骨修复中具有很大的应用潜力。
The development of biomimetic bone graft materials for periodontal tissue engineering is a field of topical interest. In this study, we designed a dual-functionalized apatite nanocomposite, which could integrate multiple molecular cues for manipulating the fate of periodontal ligament stem cells (PDLSCs). Briefly, inspired by mussels, a biomimetic nanohydroxyapatite was fabricated using a polydopamine structure as a template (named as tHA) and then surface-modified with bone-forming peptide-1 (BFP-1) and vascular endothelial growth factor-mimicking peptide (QK) via a single step of catechol chemistry. Our study showed that the biofunctions of tethered peptides were not compromised on the surface of apatite nanoparticles. Because of the synergistic effect of BFP-1 and QK peptides, the dual-functionalized apatite nanocomposite showed improved cytocompatibility compared to controls. Moreover, it can boost the proliferation and osteogenic differentiation of PDLSCs, indicating excellent bioactivity of tHA–BFP/QK nanoparticles on cell fate decision. More importantly, animal experiments showed that dual-functionalized apatite nanocomposites could dramatically promote the regeneration of periodontal bone. It is concluded that our work provides an instructive insight into the design of biomimetic apatite nanocomposites, which holds a great potential for applications in periodontal bone repair.