Gastric precancerous lesions present in Apc(Min/+) mice
Gastric precancerous lesions present in Apc(Min/+) mice
复制标题
Apc(Min/ ) 小鼠中存在胃癌前病变
DOI：
10.1016/j.biopha.2019.109534
复制
发表时间：
2020
影响因子：
7.5
通讯作者：
Wang Lijing
中科院分区：
文献类型：
--
作者：
Wang Sheng;Kuang Jianbiao;Li Guifeng;Huang Guilan;Zheng Lingyun;Li Jiangchao;Wang Lijing
ApcMin/+小鼠是家族性腺瘤性息肉病的动物模型，老年ApcMin/+小鼠也自发地在其胃中发展多个肿瘤。然而，ApcMin/+小鼠的胃癌前病变尚未得到很好的表征。用苏木精和伊红（H&E）染色和阿辛蓝染色将ApcMin/+小鼠的胃与其24周时的野生型同窝小鼠的胃进行比较。免疫组化检测Ki 67、CD 68和CA 199的表达。结果显示，前胃上皮增生和炎性浸润，胃体腺萎缩和肠上皮化生，胃窦发育不良。Apc基因突变对慢性胃炎和胃癌前病变的影响在ApcMin/+小鼠中进行了表征。这些结果表明，ApcMin/+小鼠代表了胃癌前病变的机制研究和药物发现的遗传模型。
The ApcMin/+mouse is an animal model for familial adenomatous polyposis, and aged ApcMin/+mice also spontaneously develop multiple tumors in their stomachs. However, gastric premalignant lesions in ApcMin/+mice have not been well characterized. The stomachs of ApcMin/+mice were compared with those of their wild type littermates at 24 weeks with hematoxylin and eosin (H&E) staining and alcian blue staining. Ki67, CD68 and CA199 expression was analyzed by immunohistochemistry. The results revealed the presence of epithelial proliferation and inflammatory infiltration in the forestomachs, glandular atrophy and intestinal metaplasia in the gastric bodies, and dysplasia in the gastric antra. The effect of mutations in the Apc gene on chronic gastritis and gastric precancerous lesions was characterized in ApcMin/+mice. These results suggest that ApcMin/+mice represent a genetic model for mechanistic studies and drug discovery in gastric precancerous lesions.