THE ARS CONSENSUS SEQUENCE IS REQUIRED FOR CHROMOSOMAL ORIGIN FUNCTION IN SACCHAROMYCES-CEREVISIAE
THE ARS CONSENSUS SEQUENCE IS REQUIRED FOR CHROMOSOMAL ORIGIN FUNCTION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/mcb.12.10.4305
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发表时间：
1992-10-01
影响因子：
5.3
通讯作者：
NEWLON, CS
中科院分区：
文献类型：
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作者：
DESHPANDE, AM;NEWLON, CS
复制起源已被映射到位置相吻合，在实验误差（几百个碱基对），与ARS元素。为了确定质粒上ARS功能所需的DNA序列是否是染色体起源功能所需的，缺失ARS 306的染色体拷贝，并用ARS 307的突变衍生物替换ARS 307的染色体拷贝，所述突变衍生物在ARS核心共有序列内的结构域A中含有单点突变。用双向琼脂糖凝胶电泳分析了这些衍生物的染色体起源功能。ARS306的缺失删除了相关的复制起点。结构域A突变对染色体起源功能的影响与其对ARS功能的影响一致，如通过质粒稳定性所测量的。这些结果表明，染色体起源功能是ARS元件本身的性质。
Replication origins have been mapped to positions that coincide, within experimental error (several hundred base pairs), with ARS elements. To determine whether the DNA sequences required for ARS function on plasmids are required for chromosomal origin function, the chromosomal copy of ARS306 was deleted and the chromosomal copy of ARS307 was replaced with mutant derivatives of ARS307 containing single point mutations in domain A within the ARS core consensus sequence. The chromosomal origin function of these derivatives was assayed by two-dimensional agarose gel electrophoresis. Deletion of ARS306 deleted the associated replication origin. The effects on chromosomal origin function of mutations in domain A paralleled their effects on ARS function, as measured by plasmid stability. These results demonstrate that chromosomal origin function is a property of the ARS element itself.