Genetic control of yeast mannan structure. Biochemical basis for the transformation of saccharomyces cerevisiae somatic antigen.
Genetic control of yeast mannan structure. Biochemical basis for the transformation of saccharomyces cerevisiae somatic antigen.
复制标题
酵母甘露聚糖结构的遗传控制。
DOI：
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发表时间：
1974
影响因子：
4.8
通讯作者：
C. Ballou
中科院分区：
文献类型：
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作者：
L. Rosenfeld;C. Ballou
摘要对酿酒酵母X2180磷酸甘露聚糖（甘露糖与磷酸盐的比例为38）的分析表明，它含有磷酸化的侧链，其结构为[见PDF方程]该单元显然是通过α（1 → 3）甘露糖基转移酶的作用将α（1 →3）连接的甘露糖单元添加到现有的甘露糖基磷酸甘露三糖侧链中而在甘露聚糖中产生的。推测S.酿酒酵母X2180和菌株4484- 24 D（Antalis，C.，Fogel，S.，和C. tagu，C. E.（1973）J.Biol.Chem.248，4655-4659）由该单一酶的存在或不存在引起。在其不存在的情况下，通过甘露糖磷酸转移酶的作用添加到甘露三糖侧链的甘露糖磷酸基团作为4484- 24 D甘露聚糖中的特征性抗原决定簇。然而，在菌株X2180或X2180与4484- 24 D杂交获得的杂交二倍体中，这些甘露糖基磷酸基团转化为α（1→3）-甘露二糖基磷酸基团。在所得甘露聚糖中，各种末端α（1→3）连接的甘露二糖单元赋予分子新的免疫特异性。
Abstract Analysis of Saccharomyces cerevisiae X2180 phosphomannan, with a mannose to phosphate ratio of 38, has shown that it contains phosphorylated side chains with the structure [see PDF for equation] This unit apparently arises in the mannan by the addition of α(1→3)-linked mannose units to existing mannosylphosphorylmannotriose side chains from the action of an α(1→3)mannosyltransferase. It is postulated that the immunochemical difference between S. cerevisiae X2180 and strain 4484-24D (Antalis, C., Fogel, S., and Ballou, C. E. (1973) J. Biol. Chem. 248, 4655–4659) results from the presence or absence of this single enzyme. In its absence, the mannosylphosphate groups that are added to mannotriose side chains through the action of a mannosylphosphate transferase act as characteristic antigenic determinants in the 4484-24D mannan. However, in strain X2180, or in the hybrid diploid obtained by a cross of X2180 with 4484-24D, these mannosylphosphate groups are converted to α(1→3)-mannobiosylphosphate groups. In the resulting mannan, the various terminal α(1→3)-linked mannobiose units confer on the molecule a new immunological specificity.